Synphilin-1 attenuates mutant LRRK2-induced neurodegeneration in Parkinson's disease models

Jingnan Liu, Tianxia Li, Joseph M. Thomas, Zhong Pei, Haibing Jiang, Simone Engelender, Christopher A. Ross, Wanli W. Smith

Research output: Contribution to journalArticlepeer-review

Abstract

Mutations in leucine-rich repeat kinase 2 (LRRK2) cause autosomal-dominant Parkinsonism with pleomorphic pathology including deposits of aggregated protein and neuronal degeneration. The pathogenesis of LRRK2-linked Parkinson's disease (PD) is not fully understood. Here, using co-immunoprecipitation, we found that LRRK2 interacted with synphilin-1 (SP1), a cytoplasmic protein that interacts with a-synuclein and has implications in PD pathogenesis. LRRK2 interacted with the Nterminus of SP1 whereas SP1 predominantly interacted with the C-terminus of LRRK2, including kinase domain. Co-expression of SP1 with LRRK2 increased LRRK2-induced cytoplasmic aggregation in cultured cells. Moreover, SP1 also attenuated mutant LRRK2-induced toxicity and reduced LRRK2 kinase activity in cultured cells. Knockdown of SP1 by siRNA enhanced LRRK2 neuronal toxicity. In vivo Drosophila studies, co-expression of SP1 and mutant G2019S-LRRK2 in double transgenic Drosophila increased survival and improved locomotor activity. Expression of SP1 protects against G2019S-LRRK2-induced dopamine neuron loss and reduced LRRK2 phosphorylation in double transgenic fly brains. Our findings demonstrate that SP1 attenuates mutant LRRK2-induced PD-like phenotypes and plays a neural protective role.

Original languageEnglish (US)
Pages (from-to)672-680
Number of pages9
JournalHuman molecular genetics
Volume25
Issue number4
DOIs
StatePublished - Feb 15 2016
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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