Synergistic killing effect of synthetic peptide P20 and cefotaxime on methicillin-resistant nosocomial isolates of Staphylococcus aureus

Hyun Jun Jung, Kyu Sik Choi, Dong Gun Lee

Research output: Contribution to journalArticle

Abstract

The salt resistance of antibacterial activity and synergistic effect with clinically used antibiotic agents are critical factors in developing effective peptide antibiotic drugs. For this reason, we investigated the resistance of antibacterial activity to antagonism induced by NaCl and MgCl2 and the synergistic effect of P20 with cefotaxime. P20 is a 20-residue synthetic peptide derived from a cecropin A (CA)-melittin (ME) hybrid peptide. In this study, P20 was found to have potent antibacterial activity against clinically isolated methicillin-resistant Staphylococcus aureus (MRSA) strains without hemolytic activity against human erythrocytes. The combination study revealed that P20 in combination with cefotaxime showed synergistic antibacterial activity in an energy-dependent manner. We also confirmed the synergism between P20 and cefotaxime by fluorescence-activated flow cytometric analysis by staining bacterial cells with propidium iodide (PI) and bis-(1,3-dibutylbarbituric acid) trimethine oxonol (BOX). This study suggests that P20 may be useful as a therapeutic antibiotic peptide with synergistic effect in combination with conventional antibiotic agents.

Original languageEnglish (US)
Pages (from-to)1039-1046
Number of pages8
JournalJournal of Microbiology and Biotechnology
Volume15
Issue number5
StatePublished - Oct 1 2005
Externally publishedYes

Keywords

  • Antibacterial activity
  • Cefotaxime
  • Hybrid peptide
  • Methicillin-resistant Staphylococcus aureus (MRSA)
  • Salts resistance
  • Synergistic effect

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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