@article{a6c7f379b745470eb6f618c4dee80576,
title = "Synergistic Coordination of Chromatin Torsional Mechanics and Topoisomerase Activity",
abstract = "The material properties of eukaryotic chromatin fibers partition supercoiling ahead of progressing replication forks, illustrating that chromatin provides a buffer against torsional stress and that its unique mechanical properties help to facilitate replication and minimize genome instability.",
keywords = "DNA supercoiling, angular optical trap, braiding, chromatin, fork rotation, magnetic tweezers, replication, topoisomerase, torque, torsional modulus",
author = "Le, {Tung T.} and Xiang Gao and Park, {Seong ha} and Jaeyoon Lee and Inman, {James T.} and Lee, {Joyce H.} and Killian, {Jessica L.} and Badman, {Ryan P.} and Berger, {James M.} and Wang, {Michelle D.}",
note = "Funding Information: We thank members of the Wang Laboratory and J.P. Sethna, D.B. Liarte, V. Gahdi, E.E. Alani, and J.E. Peters for helpful discussion and comments; J.E. Baker for initial data analysis software of the MT; and L.D. Brennan for purification of histones. This work was supported by HHMI (to M.D.W.) and NIH (T32GM008267 to M.D.W. F31CA224896 to J.H.L. and R01CA077373 to J.M.B.). The quartz cylinder fabrication was performed at the Cornell NanoScale Science & Technology Facility (CNF), a member of the National Nanotechnology Coordinated Infrastructure (NNCI), which is supported by NSF (NNCI-1542081). T.T.L. X.G. S.P. J.L. J.T.I. and M.D.W. designed single molecule assays. T.T.L. X.G. S.P. and J.L. performed experiments. T.T.L. X.G. and M.D.W. analyzed data. S.P. and T.T.L. prepared the DNA construct. T.T.L. optimized single molecule assays and prepared essential reagents. X.G. and J.T.I. upgraded the AOT. R.P.B. nanofabricated the quartz cylinders. J.T.I. constructed the MT with contribution from J.L.K. on software. J.H.L. and J.M.B. purified topo II and characterized it biochemically. M.D.W. and J.L.K. wrote the initial draft, and all authors contributed to manuscript revisions. M.D.W. supervised the project. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = oct,
day = "17",
doi = "10.1016/j.cell.2019.09.034",
language = "English (US)",
volume = "179",
pages = "619--631.e15",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "3",
}