Synaptic vesicular monoamine transporter expression: distribution and pharmacologic profile

Antonio M. Gonzalez, Donna Walther, Angel Pazos, George R. Uhl

Research output: Contribution to journalArticlepeer-review

Abstract

The human vesicular monoamine transporter (hSVMT) cDNA predicts a protein of 515 amino acids that shares 92% amino acid identity with the rat cDNA. Northern analyses reveal expression of 4.3 kb SVMT mRNAs in rat hypothalamus, midbrain and brainstem, a 3 kb hSVMT mRNA in human brainstem and a 4.8 kb hSVMT mRNA in human hypothalamus. In situ hybridization documents significant SVMT expression in human nigra compacta neurons and in rat hypothalamic neurons whose distribution patterns are identical to those previously reported to display histaminergic markers. COS cell hSVMT expression yielded nanomolar affinities for tetrabenazine and reserpine, micromolar affinities for haloperidol, GBR12909, serotonin, mazindol, nomifensin and d-amphetamine, while dopamine, epinephrine, norepinephrine and histamine each displayed millimolar affinities. These observations extend the pharmacological characterization of hSVMT and studies of its distribution, and indicate likely physiological roles for SVMT in packaging monoamine transmitters including histamine.

Original languageEnglish (US)
Pages (from-to)219-226
Number of pages8
JournalMolecular Brain Research
Volume22
Issue number1-4
DOIs
StatePublished - 1994

Keywords

  • Dihydrotetrahydrobenazine
  • Dopamine
  • Epinephrine
  • Monoamine transporter
  • Norepinephrine
  • Reserpine
  • Serotonin
  • Synaptic vesicle

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Fingerprint

Dive into the research topics of 'Synaptic vesicular monoamine transporter expression: distribution and pharmacologic profile'. Together they form a unique fingerprint.

Cite this