Synaptic interaction between GABAergic terminals and substance P receptor-positive neurons in rat spinal superficial laminae

Y. X. Tao, Y. Q. Li, Z. Q. Zhao

Research output: Contribution to journalArticlepeer-review

Abstract

AIM: To identify synaptic relationship between γ-aminobutyric acid (GABA)-containing terminals and substance P receptor-positive neurons in the superficial dorsal horn of rat spinal cord. METHODS: A combination of preembedding immunoperoxidase staining for substance P receptor with postembedding immunogold staining for GABA was employed. RESULTS: Intense substance P receptor-expressing neurons were concentrated in medial half of the lamina I and the outer lamina II of the spinal cord. Under electron microscopy, substance P receptor immunoreactivity was found mainly in perikarya and dendrites, and deposited along neuronal membranes, in Golgi complex, in rough endoplasmic reticulum and on the surface of mitochondria with patch- or granular-like staining. The double-labeling studies revealed that substance P receptor-expressing dendrites received symmetric synaptic contacts from axonal terminals labeled with immunogold particles indicating GABA. GABA also co-localized with substance P receptors in the dendrites. CONCLUSION: The synaptic contact between GABA-containing terminal and substance P receptor-expressing neuron provided important morphological evidence for previous pharmacological studies concerning antinociceptive function of GABA in the spinal cord.

Original languageEnglish (US)
Pages (from-to)911-914
Number of pages4
JournalActa Pharmacologica Sinica
Volume21
Issue number10
StatePublished - Jan 1 2000

Keywords

  • Colocalization
  • GABA
  • Spinal cord
  • Substance P receptors
  • Synaptic transmission

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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