Synaptic activity-induced conversion of intronic to exonic sequence in Homer 1 immediate early gene expression

Daniele Bottai, John F. Guzowski, Martin K. Schwarz, Shin H. Kang, Bo Xiao, Anthony Lanahan, Paul F Worley, Peter H. Seeburg

Research output: Contribution to journalArticle

Abstract

Three Homer genes regulate the activity of metabotropic glutamate receptors mGluR1a and mGluR5 and their coupling to releasable intracellular Ca2+ pools and ion channels. Only the Homer 1 gene evolved bimodal expression of constitutive (Homer 1b and c) and immediate early gene (IEG) products (Homer 1a and Ania 3). The IEG forms compete functionally with the constitutive Homer proteins. The complex expression of the Homer 1 gene, unique for IEGs, focused our attention on the gene organization. In contrast to most IEGs, which have genes that are 10-fold activity-dependent increase in mRNA levels exclusively for the IEG forms. Moreover, fluorescent in situ hybridization documented that new primary Homer 1 transcripts are induced in neuronal nuclei within a few minutes after seizure, typical of IEGs, and that Homer 1b-specific exons are excluded from the activity-induced transcripts. Thus, at the resting state of the neurons, the entire gene is constitutively transcribed at low levels to yield Homer 1b/c transcripts. Neuronal activity sharply increases the rate of transcription initiation, with most transcripts now ending within the central intron. These coordinate transcriptional events rapidly convert a constitutive gene to an IEG and regulate the expression of functionally different Homer 1 proteins.

Original languageEnglish (US)
Pages (from-to)167-175
Number of pages9
JournalJournal of Neuroscience
Volume22
Issue number1
StatePublished - Jan 1 2002

Fingerprint

Immediate-Early Genes
Gene Expression
Genes
Metabotropic Glutamate Receptors
Homer Scaffolding Proteins
Fluorescence In Situ Hybridization
Ion Channels
Introns
Exons
Seizures
Neurons
Messenger RNA

Keywords

  • Activity-dependent switch of intron to exon
  • Alternative splicing
  • Alternative transcriptional termination
  • Fluorescent in situ hybridization
  • Immediate early gene
  • Induced neuronal activity
  • RNase protection

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Bottai, D., Guzowski, J. F., Schwarz, M. K., Kang, S. H., Xiao, B., Lanahan, A., ... Seeburg, P. H. (2002). Synaptic activity-induced conversion of intronic to exonic sequence in Homer 1 immediate early gene expression. Journal of Neuroscience, 22(1), 167-175.

Synaptic activity-induced conversion of intronic to exonic sequence in Homer 1 immediate early gene expression. / Bottai, Daniele; Guzowski, John F.; Schwarz, Martin K.; Kang, Shin H.; Xiao, Bo; Lanahan, Anthony; Worley, Paul F; Seeburg, Peter H.

In: Journal of Neuroscience, Vol. 22, No. 1, 01.01.2002, p. 167-175.

Research output: Contribution to journalArticle

Bottai, D, Guzowski, JF, Schwarz, MK, Kang, SH, Xiao, B, Lanahan, A, Worley, PF & Seeburg, PH 2002, 'Synaptic activity-induced conversion of intronic to exonic sequence in Homer 1 immediate early gene expression', Journal of Neuroscience, vol. 22, no. 1, pp. 167-175.
Bottai D, Guzowski JF, Schwarz MK, Kang SH, Xiao B, Lanahan A et al. Synaptic activity-induced conversion of intronic to exonic sequence in Homer 1 immediate early gene expression. Journal of Neuroscience. 2002 Jan 1;22(1):167-175.
Bottai, Daniele ; Guzowski, John F. ; Schwarz, Martin K. ; Kang, Shin H. ; Xiao, Bo ; Lanahan, Anthony ; Worley, Paul F ; Seeburg, Peter H. / Synaptic activity-induced conversion of intronic to exonic sequence in Homer 1 immediate early gene expression. In: Journal of Neuroscience. 2002 ; Vol. 22, No. 1. pp. 167-175.
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