Sympathetic innervation during development is necessary for pancreatic islet architecture and functional maturation

Philip Borden, Jessica Houtz, Steven D. Leach, Rejji Kuruvilla

Research output: Contribution to journalArticle

Abstract

Sympathetic neurons depend on target-derived neurotrophic cues to control their survival and growth. However, whether sympathetic innervation contributes reciprocally to the development of target tissues is less clear. Here, we report that sympathetic innervation is necessary for the formation of the pancreatic islets of Langerhans and for their functional maturation. Genetic or pharmacological ablation of sympathetic innervation during development resulted in altered islet architecture, reduced insulin secretion, and impaired glucose tolerance in mice. Similar defects were observed with pharmacological blockade of β-adrenergic signaling. Conversely, the administration of a β-adrenergic agonist restored islet morphology and glucose tolerance in deinnervated animals. Furthermore, in neuron-islet cocultures, sympathetic neurons promoted islet cell migration in a β-adrenergic-dependent manner. This study reveals that islet architecture requires extrinsic inductive cues from neighboring tissues such as sympathetic nerves and suggests that early perturbations in sympathetic innervation might underlie metabolic disorders

Original languageEnglish (US)
Pages (from-to)287-301
Number of pages15
JournalCell Reports
Volume4
Issue number2
DOIs
StatePublished - Jul 25 2013

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Islets of Langerhans
Neurons
Adrenergic Agents
Cues
Pharmacology
Tissue
Glucose
Adrenergic Agonists
Glucose Intolerance
Coculture Techniques
Ablation
Cell Movement
Animals
Insulin
Defects
Growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Sympathetic innervation during development is necessary for pancreatic islet architecture and functional maturation. / Borden, Philip; Houtz, Jessica; Leach, Steven D.; Kuruvilla, Rejji.

In: Cell Reports, Vol. 4, No. 2, 25.07.2013, p. 287-301.

Research output: Contribution to journalArticle

Borden, Philip ; Houtz, Jessica ; Leach, Steven D. ; Kuruvilla, Rejji. / Sympathetic innervation during development is necessary for pancreatic islet architecture and functional maturation. In: Cell Reports. 2013 ; Vol. 4, No. 2. pp. 287-301.
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