Symbiotic characterization of isoleucine + valine and leucine auxotrophs of Sinorhizobium meliloti

Raad Hassani, C. Krishna Prasad, K. E. Vineetha, Neeraj Vij, Prachi Singh, Reeteka Sud, Sangeeta Yadav, Gursharn S. Randhawa

Research output: Contribution to journalArticle

Abstract

Ten isoleucine+valine and three leucine auxotrophs of Sinorhizobium meliloti Rmd201 were obtained by random mutagenesis with transposon Tn5 followed by screening of Tn5 derivatives on minimal medium supplemented with modified Holliday pools. Based on intermediate feeding, intermediate accumulation and cross-feeding studies, isoleucine+valine and leucine auxotrophs were designated as ilvB/ilvG, ilvC and ilvD, and leuC/leuD and leuB mutants, respectively. Symbiotic properties of all ilvD mutants with alfalfa plants were similar to those of the parental strain. The ilvB/ilvG and ilvC mutants were Nod -, Inoculation of alfalfa plants with ilvB/ilvG mutant did not result in root hair curling and infection thread formation. The ilvC mutants were capable of curling root hairs but did not induce infection thread formation. All leucine auxotrophs were Nod+ Fix -. Supplementation of leucine to the plant nutrient medium did not restore symbiotic effectiveness to the auxotrophs. Histological studies revealed that the nodules induced by the leucine auxotrophs did not develop fully like those induced by the parental strain. The nodules induced by leuB mutants were structurally more advanced than the leuC/leuD mutant induced nodules. These results indicate that ilvB/ilvG, ilvC and one or two leu genes of S. meliloti may have a role in symbiosis. The position of ilv genes on the chromosomal map of S. meliloti was found to be near ade-15 marker.

Original languageEnglish (US)
Pages (from-to)1110-1120
Number of pages11
JournalIndian Journal of Experimental Biology
Volume40
Issue number10
StatePublished - Oct 1 2002
Externally publishedYes

Fingerprint

Sinorhizobium meliloti
Isoleucine
Valine
Leucine
Genes
Mutagenesis
Medicago sativa
Nutrients
Screening
Derivatives
Gene Order
Symbiosis
Infection
Food

ASJC Scopus subject areas

  • Bioengineering

Cite this

Hassani, R., Krishna Prasad, C., Vineetha, K. E., Vij, N., Singh, P., Sud, R., ... Randhawa, G. S. (2002). Symbiotic characterization of isoleucine + valine and leucine auxotrophs of Sinorhizobium meliloti. Indian Journal of Experimental Biology, 40(10), 1110-1120.

Symbiotic characterization of isoleucine + valine and leucine auxotrophs of Sinorhizobium meliloti. / Hassani, Raad; Krishna Prasad, C.; Vineetha, K. E.; Vij, Neeraj; Singh, Prachi; Sud, Reeteka; Yadav, Sangeeta; Randhawa, Gursharn S.

In: Indian Journal of Experimental Biology, Vol. 40, No. 10, 01.10.2002, p. 1110-1120.

Research output: Contribution to journalArticle

Hassani, R, Krishna Prasad, C, Vineetha, KE, Vij, N, Singh, P, Sud, R, Yadav, S & Randhawa, GS 2002, 'Symbiotic characterization of isoleucine + valine and leucine auxotrophs of Sinorhizobium meliloti', Indian Journal of Experimental Biology, vol. 40, no. 10, pp. 1110-1120.
Hassani R, Krishna Prasad C, Vineetha KE, Vij N, Singh P, Sud R et al. Symbiotic characterization of isoleucine + valine and leucine auxotrophs of Sinorhizobium meliloti. Indian Journal of Experimental Biology. 2002 Oct 1;40(10):1110-1120.
Hassani, Raad ; Krishna Prasad, C. ; Vineetha, K. E. ; Vij, Neeraj ; Singh, Prachi ; Sud, Reeteka ; Yadav, Sangeeta ; Randhawa, Gursharn S. / Symbiotic characterization of isoleucine + valine and leucine auxotrophs of Sinorhizobium meliloti. In: Indian Journal of Experimental Biology. 2002 ; Vol. 40, No. 10. pp. 1110-1120.
@article{c0f574a857f3478da9fba4cc9cd27fc4,
title = "Symbiotic characterization of isoleucine + valine and leucine auxotrophs of Sinorhizobium meliloti",
abstract = "Ten isoleucine+valine and three leucine auxotrophs of Sinorhizobium meliloti Rmd201 were obtained by random mutagenesis with transposon Tn5 followed by screening of Tn5 derivatives on minimal medium supplemented with modified Holliday pools. Based on intermediate feeding, intermediate accumulation and cross-feeding studies, isoleucine+valine and leucine auxotrophs were designated as ilvB/ilvG, ilvC and ilvD, and leuC/leuD and leuB mutants, respectively. Symbiotic properties of all ilvD mutants with alfalfa plants were similar to those of the parental strain. The ilvB/ilvG and ilvC mutants were Nod -, Inoculation of alfalfa plants with ilvB/ilvG mutant did not result in root hair curling and infection thread formation. The ilvC mutants were capable of curling root hairs but did not induce infection thread formation. All leucine auxotrophs were Nod+ Fix -. Supplementation of leucine to the plant nutrient medium did not restore symbiotic effectiveness to the auxotrophs. Histological studies revealed that the nodules induced by the leucine auxotrophs did not develop fully like those induced by the parental strain. The nodules induced by leuB mutants were structurally more advanced than the leuC/leuD mutant induced nodules. These results indicate that ilvB/ilvG, ilvC and one or two leu genes of S. meliloti may have a role in symbiosis. The position of ilv genes on the chromosomal map of S. meliloti was found to be near ade-15 marker.",
author = "Raad Hassani and {Krishna Prasad}, C. and Vineetha, {K. E.} and Neeraj Vij and Prachi Singh and Reeteka Sud and Sangeeta Yadav and Randhawa, {Gursharn S.}",
year = "2002",
month = "10",
day = "1",
language = "English (US)",
volume = "40",
pages = "1110--1120",
journal = "Journal of scientific & industrial research. C. Biological sciences",
issn = "0019-5189",
publisher = "National Institute of Science Communication",
number = "10",

}

TY - JOUR

T1 - Symbiotic characterization of isoleucine + valine and leucine auxotrophs of Sinorhizobium meliloti

AU - Hassani, Raad

AU - Krishna Prasad, C.

AU - Vineetha, K. E.

AU - Vij, Neeraj

AU - Singh, Prachi

AU - Sud, Reeteka

AU - Yadav, Sangeeta

AU - Randhawa, Gursharn S.

PY - 2002/10/1

Y1 - 2002/10/1

N2 - Ten isoleucine+valine and three leucine auxotrophs of Sinorhizobium meliloti Rmd201 were obtained by random mutagenesis with transposon Tn5 followed by screening of Tn5 derivatives on minimal medium supplemented with modified Holliday pools. Based on intermediate feeding, intermediate accumulation and cross-feeding studies, isoleucine+valine and leucine auxotrophs were designated as ilvB/ilvG, ilvC and ilvD, and leuC/leuD and leuB mutants, respectively. Symbiotic properties of all ilvD mutants with alfalfa plants were similar to those of the parental strain. The ilvB/ilvG and ilvC mutants were Nod -, Inoculation of alfalfa plants with ilvB/ilvG mutant did not result in root hair curling and infection thread formation. The ilvC mutants were capable of curling root hairs but did not induce infection thread formation. All leucine auxotrophs were Nod+ Fix -. Supplementation of leucine to the plant nutrient medium did not restore symbiotic effectiveness to the auxotrophs. Histological studies revealed that the nodules induced by the leucine auxotrophs did not develop fully like those induced by the parental strain. The nodules induced by leuB mutants were structurally more advanced than the leuC/leuD mutant induced nodules. These results indicate that ilvB/ilvG, ilvC and one or two leu genes of S. meliloti may have a role in symbiosis. The position of ilv genes on the chromosomal map of S. meliloti was found to be near ade-15 marker.

AB - Ten isoleucine+valine and three leucine auxotrophs of Sinorhizobium meliloti Rmd201 were obtained by random mutagenesis with transposon Tn5 followed by screening of Tn5 derivatives on minimal medium supplemented with modified Holliday pools. Based on intermediate feeding, intermediate accumulation and cross-feeding studies, isoleucine+valine and leucine auxotrophs were designated as ilvB/ilvG, ilvC and ilvD, and leuC/leuD and leuB mutants, respectively. Symbiotic properties of all ilvD mutants with alfalfa plants were similar to those of the parental strain. The ilvB/ilvG and ilvC mutants were Nod -, Inoculation of alfalfa plants with ilvB/ilvG mutant did not result in root hair curling and infection thread formation. The ilvC mutants were capable of curling root hairs but did not induce infection thread formation. All leucine auxotrophs were Nod+ Fix -. Supplementation of leucine to the plant nutrient medium did not restore symbiotic effectiveness to the auxotrophs. Histological studies revealed that the nodules induced by the leucine auxotrophs did not develop fully like those induced by the parental strain. The nodules induced by leuB mutants were structurally more advanced than the leuC/leuD mutant induced nodules. These results indicate that ilvB/ilvG, ilvC and one or two leu genes of S. meliloti may have a role in symbiosis. The position of ilv genes on the chromosomal map of S. meliloti was found to be near ade-15 marker.

UR - http://www.scopus.com/inward/record.url?scp=0036795495&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036795495&partnerID=8YFLogxK

M3 - Article

VL - 40

SP - 1110

EP - 1120

JO - Journal of scientific & industrial research. C. Biological sciences

JF - Journal of scientific & industrial research. C. Biological sciences

SN - 0019-5189

IS - 10

ER -