Sustained expression of lipocalin-2 during polymicrobial sepsis

Daniel E. Vazquez, Diego Nino, Antonio De Maio, David M. Cauvi

Research output: Contribution to journalArticle

Abstract

Sepsis is a major healthcare problem and a leading cause of death worldwide. There is no dependable diagnosis, and treatment for this condition remains mainly supportive. The etiology of sepsis is related to an overwhelming inflammatory response. In this regard, the antimicrobial protein lipocalin-2 (Lcn2) has been associated with several inflammatory conditions, but its contribution to polymicrobial sepsis is unclear. Polymicrobial sepsis was induced by cecal ligation and puncture (CLP), and Lcn2 mRNA levels and protein expression were measured in liver and lung tissues. We observed that Lcn2 expression was robustly induced in liver and lung of C57BL/6 J (B6) mice, and remained elevated during the stage of innate immune dysfunction observed in sepsis. This response was different in A/J mice, suggesting a contribution of the genetic background, probably due to differences in IL-10 expression between these two mouse strains. Indeed, IL-10 was found to regulate Lcn2 expression in both primary and J774A.1 macrophages. Thus, Lcn2 expression is highly regulated during CLP-induced sepsis, suggesting that this antimicrobial protein could have a role as a potential biomarker for the diagnosis of sepsis.

Original languageEnglish (US)
Pages (from-to)477-489
Number of pages13
JournalInnate Immunity
Volume21
Issue number5
DOIs
StatePublished - Jul 4 2015
Externally publishedYes

Fingerprint

Sepsis
Punctures
Interleukin-10
Ligation
Lung
Liver
Lipocalin-2
Cause of Death
Proteins
Biomarkers
Macrophages
Delivery of Health Care
Messenger RNA

Keywords

  • animal model
  • innate immunity
  • lipocalin-2
  • macrophages
  • Sepsis

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Immunology
  • Microbiology
  • Infectious Diseases
  • Medicine(all)

Cite this

Sustained expression of lipocalin-2 during polymicrobial sepsis. / Vazquez, Daniel E.; Nino, Diego; De Maio, Antonio; Cauvi, David M.

In: Innate Immunity, Vol. 21, No. 5, 04.07.2015, p. 477-489.

Research output: Contribution to journalArticle

Vazquez, Daniel E. ; Nino, Diego ; De Maio, Antonio ; Cauvi, David M. / Sustained expression of lipocalin-2 during polymicrobial sepsis. In: Innate Immunity. 2015 ; Vol. 21, No. 5. pp. 477-489.
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