Susceptibility to apoptosis is enhanced in immature cortical neurons

John W. McDonald; Maria I. Behrens; Chris Chung; Tim Bhattacharyya; Dennis W. Choi

doi:10.1016/S0006-8993(97)00248-5

Susceptibility to apoptosis is enhanced in immature cortical neurons

John W. McDonald, Maria I. Behrens, Chris Chung, Tim Bhattacharyya, Dennis W. Choi

School of Medicine

Research output: Contribution to journal › Article › peer-review

89 Scopus citations

Abstract

The susceptibility of cortical neurons to two forms of apoptotic death was compared with susceptibility to excitotoxic death during development in vitro (DIV 4-21). Murine cortical cultures were exposed for 48 h to the phosphatase inhibitor cyclosporine, the protein kinase inhibitor staurosporine or the excitotoxin N-methyl-D-aspartate (NMDA). Susceptibility to apoptosis induced by staurosporine or cyclosporine was maximal between DIV 4-10 and declined from DIV 10 through 18. The opposite pattern was observed with susceptibility to NMDA receptor-mediated excitotoxic necrosis, which was minimal at DIV 6 and progressively increased through DIV 21.

Original language	English (US)
Pages (from-to)	228-232
Number of pages	5
Journal	Brain research
Volume	759
Issue number	2
DOIs	https://doi.org/10.1016/S0006-8993(97)00248-5
State	Published - Jun 6 1997

Keywords

Aptoptosis
Cyclosporine
Development
Excitotoxicity
N- Methyl-D-aspartate (NMDA)
Neuronal culture

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(97)00248-5

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@article{640e20edf2d146fe8c9eacadb6410394,

title = "Susceptibility to apoptosis is enhanced in immature cortical neurons",

abstract = "The susceptibility of cortical neurons to two forms of apoptotic death was compared with susceptibility to excitotoxic death during development in vitro (DIV 4-21). Murine cortical cultures were exposed for 48 h to the phosphatase inhibitor cyclosporine, the protein kinase inhibitor staurosporine or the excitotoxin N-methyl-D-aspartate (NMDA). Susceptibility to apoptosis induced by staurosporine or cyclosporine was maximal between DIV 4-10 and declined from DIV 10 through 18. The opposite pattern was observed with susceptibility to NMDA receptor-mediated excitotoxic necrosis, which was minimal at DIV 6 and progressively increased through DIV 21.",

keywords = "Aptoptosis, Cyclosporine, Development, Excitotoxicity, N- Methyl-D-aspartate (NMDA), Neuronal culture",

author = "McDonald, {John W.} and Behrens, {Maria I.} and Chris Chung and Tim Bhattacharyya and Choi, {Dennis W.}",

note = "Funding Information: This work was supported by National Institute of Health, National Institute of Neurological Disorders and Stroke Grants NS01931 (to J.W.McD.), and NS32636 and NS30337 (to D.W.C.). Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "1997",

month = jun,

day = "6",

doi = "10.1016/S0006-8993(97)00248-5",

language = "English (US)",

volume = "759",

pages = "228--232",

journal = "Brain Research",

issn = "0006-8993",

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number = "2",

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TY - JOUR

T1 - Susceptibility to apoptosis is enhanced in immature cortical neurons

AU - McDonald, John W.

AU - Behrens, Maria I.

AU - Chung, Chris

AU - Bhattacharyya, Tim

AU - Choi, Dennis W.

N1 - Funding Information: This work was supported by National Institute of Health, National Institute of Neurological Disorders and Stroke Grants NS01931 (to J.W.McD.), and NS32636 and NS30337 (to D.W.C.). Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 1997/6/6

Y1 - 1997/6/6

N2 - The susceptibility of cortical neurons to two forms of apoptotic death was compared with susceptibility to excitotoxic death during development in vitro (DIV 4-21). Murine cortical cultures were exposed for 48 h to the phosphatase inhibitor cyclosporine, the protein kinase inhibitor staurosporine or the excitotoxin N-methyl-D-aspartate (NMDA). Susceptibility to apoptosis induced by staurosporine or cyclosporine was maximal between DIV 4-10 and declined from DIV 10 through 18. The opposite pattern was observed with susceptibility to NMDA receptor-mediated excitotoxic necrosis, which was minimal at DIV 6 and progressively increased through DIV 21.

AB - The susceptibility of cortical neurons to two forms of apoptotic death was compared with susceptibility to excitotoxic death during development in vitro (DIV 4-21). Murine cortical cultures were exposed for 48 h to the phosphatase inhibitor cyclosporine, the protein kinase inhibitor staurosporine or the excitotoxin N-methyl-D-aspartate (NMDA). Susceptibility to apoptosis induced by staurosporine or cyclosporine was maximal between DIV 4-10 and declined from DIV 10 through 18. The opposite pattern was observed with susceptibility to NMDA receptor-mediated excitotoxic necrosis, which was minimal at DIV 6 and progressively increased through DIV 21.

KW - Aptoptosis

KW - Cyclosporine

KW - Development

KW - Excitotoxicity

KW - N- Methyl-D-aspartate (NMDA)

KW - Neuronal culture

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U2 - 10.1016/S0006-8993(97)00248-5

DO - 10.1016/S0006-8993(97)00248-5

M3 - Article

C2 - 9221941

AN - SCOPUS:0030743984

VL - 759

SP - 228

EP - 232

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -

Susceptibility to apoptosis is enhanced in immature cortical neurons

Abstract

Keywords

ASJC Scopus subject areas

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