Susceptibility of adherent versus suspension target cells derived from adherent tissue culture lines to cell-mediated cytotoxicity in rapid 51Cr-release assays

N. A. Dorfman, C. I. Civin, J. R. Wunderlich

Research output: Contribution to journalArticle

Abstract

Preparation of target cells from tissue culture lines which grow adherent to tissue culture vessels is often desirable for tests of cell-mediated cytotoxicity (CMC). In the present study we used cells derived from adherent tissue culture lines to compare the merits of suspension vs. adherent target cells in short-term 51Cr-release assays. Cytotoxic activity of murine spleen cells sensitized in vitro against allogeneic spleen cells or syngeneic sarcoma cells was tested with fibroblast or sarcoma target cells. In parallel tests, aliquots of tissue culture lines were detached and used as either suspension or adherent target cells in CMC assays, matching the concentrations of suspension and adherent target cells. In both allogeneic and syngeneic combinations adherent target cells released less 51Cr spontaneously and were more susceptible to CMC than their suspension counterparts.

Original languageEnglish (US)
Pages (from-to)127-139
Number of pages13
JournalJournal of Immunological Methods
Volume32
Issue number2
DOIs
StatePublished - Jan 28 1980
Externally publishedYes

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ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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