Surprising absence of heparin in the intestinal mucosa of baby pigs

Yanlei Yu, Yin Chen, Paiyz Mikael, Fuming Zhang, Apryll M. Stalcup, Rebecca German, Francois Gould, Jocelyn Ohlemacher, Hong Zhang, Robert J. Linhardt

Research output: Contribution to journalArticle

Abstract

Heparin, a member of a family of molecules called glycosaminoglycans, is biosynthesized in mucosal mast cells. This important anticoagulant polysaccharide is primarily produced by extraction of the mast cell-rich intestinal mucosa of hogs. There is concern about our continued ability to supply sufficient heparin to support the worldwide growth of advanced medical procedures from the static population of adult hogs used as food animals. While the intestinal mucosa of adult pigs is rich in anticoagulant heparin (containing a few hundred milligrams per animal), little is known about how the content of heparin changes with animal age. Using sophisticated mass spectral analysis we discovered that heparin was largely absent from the intestinal mucosa of piglets. Moreover, while the related, nonanticoagulant heparan sulfate glycosaminoglycan was present in significant amounts we found little chondroitin sulfate E also associated with mast cells. Histological evaluation of piglet intestinal mucosa showed a very low mast cell content. Respiratory mast cells have been reported in baby pigs suggesting that there was something unique about the piglets used in the current study. These piglets were raised in the relatively clean environment of a university animal facility and treated with antibiotics over their lifetime resulting in a depleted microbiome that greatly reduced the number of mast cells and heparin content of the intestinal mucosal in these animals. Thus, from the current study it remains unclear whether the lack of intestinal mast cellderived heparin results from the young age of these animals or their exposure to their depleted microbiome.

Original languageEnglish (US)
Pages (from-to)57-63
Number of pages7
JournalGlycobiology
Volume27
Issue number1
DOIs
StatePublished - 2017

Fingerprint

Intestinal Mucosa
Heparin
Mast Cells
Swine
Animals
Microbiota
Glycosaminoglycans
Anticoagulants
Gastrointestinal Contents
Aptitude
Heparitin Sulfate
Chondroitin Sulfates
Mucous Membrane
Spectrum analysis
Polysaccharides
Anti-Bacterial Agents
Food
Molecules
Growth
Population

Keywords

  • Antibiotics
  • Heparin
  • Intestine
  • Microbiome
  • Pig

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yu, Y., Chen, Y., Mikael, P., Zhang, F., Stalcup, A. M., German, R., ... Linhardt, R. J. (2017). Surprising absence of heparin in the intestinal mucosa of baby pigs. Glycobiology, 27(1), 57-63. https://doi.org/10.1093/glycob/cww104

Surprising absence of heparin in the intestinal mucosa of baby pigs. / Yu, Yanlei; Chen, Yin; Mikael, Paiyz; Zhang, Fuming; Stalcup, Apryll M.; German, Rebecca; Gould, Francois; Ohlemacher, Jocelyn; Zhang, Hong; Linhardt, Robert J.

In: Glycobiology, Vol. 27, No. 1, 2017, p. 57-63.

Research output: Contribution to journalArticle

Yu, Y, Chen, Y, Mikael, P, Zhang, F, Stalcup, AM, German, R, Gould, F, Ohlemacher, J, Zhang, H & Linhardt, RJ 2017, 'Surprising absence of heparin in the intestinal mucosa of baby pigs', Glycobiology, vol. 27, no. 1, pp. 57-63. https://doi.org/10.1093/glycob/cww104
Yu Y, Chen Y, Mikael P, Zhang F, Stalcup AM, German R et al. Surprising absence of heparin in the intestinal mucosa of baby pigs. Glycobiology. 2017;27(1):57-63. https://doi.org/10.1093/glycob/cww104
Yu, Yanlei ; Chen, Yin ; Mikael, Paiyz ; Zhang, Fuming ; Stalcup, Apryll M. ; German, Rebecca ; Gould, Francois ; Ohlemacher, Jocelyn ; Zhang, Hong ; Linhardt, Robert J. / Surprising absence of heparin in the intestinal mucosa of baby pigs. In: Glycobiology. 2017 ; Vol. 27, No. 1. pp. 57-63.
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