Surface supported bilayer platform for studies of lateral association of proteins in membranes

Mikhail Merzlyakov, Edwin Li, Kalina A Hristova

Research output: Contribution to journalArticle

Abstract

Here, the authors review how surface supported bilayers can be engineered and how Förster resonance energy transfer (FRET) can be used to quantify interactions between transmembrane peptides in these bilayers. The requirements for the surface supported platform are (1) lateral mobility of the peptides, (2) transmembrane orientation of the peptides, and (3) capabilities for FRET measurements. To satisfy these requirements, a new assembly method, termed "directed assembly" was developed. This assembly method could have broad utility in basic studies of proteins in membranes and in biotechnological applications.

Original languageEnglish (US)
JournalBiointerphases
Volume3
Issue number2
DOIs
StatePublished - Jun 2008

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Peptides
peptides
Membrane Proteins
platforms
assembly
Energy Transfer
membranes
proteins
Proteins
Membranes
Energy transfer
energy transfer
requirements
interactions

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Surface supported bilayer platform for studies of lateral association of proteins in membranes. / Merzlyakov, Mikhail; Li, Edwin; Hristova, Kalina A.

In: Biointerphases, Vol. 3, No. 2, 06.2008.

Research output: Contribution to journalArticle

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