Surface supported bilayer platform for studies of lateral association of proteins in membranes

Mikhail Merzlyakov, Edwin Li, Kalina Hristova

Research output: Contribution to journalArticle

Abstract

Here, the authors review how surface supported bilayers can be engineered and how Förster resonance energy transfer (FRET) can be used to quantify interactions between transmembrane peptides in these bilayers. The requirements for the surface supported platform are (1) lateral mobility of the peptides, (2) transmembrane orientation of the peptides, and (3) capabilities for FRET measurements. To satisfy these requirements, a new assembly method, termed "directed assembly" was developed. This assembly method could have broad utility in basic studies of proteins in membranes and in biotechnological applications.

Original languageEnglish (US)
Pages (from-to)FA80-FA84
JournalBiointerphases
Volume3
Issue number2
DOIs
StatePublished - Jun 2008

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ASJC Scopus subject areas

  • Chemistry(all)
  • Biomaterials
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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