Surface immunoglobulin isotype, activation, and tolerance susceptibility of B lymphocytes of New Zealand black mice

B. L. Pike, J. E. Layton, N. R. Rose, G. J V Nossal

Research output: Contribution to journalArticle

Abstract

Fluorescence-activated cell sorter (FACS) analysis of B-lymphocyte surface isotype expression, and limiting dilution B-lymphocyte cloning techniques, have been used to establish characteristics of B lymphocytes from New Zealand Black (NZB) mice which might contribute to the predisposition of this strain to autoimmune disease. The NZB mice and two other strains (BALB/c and CBA) used were either specific pathogen free (SPF) or germ free (GF). The NZB B lymphocytes differed from the normal strains in the following respects: they showed considerably higher spontaneous conversion into antibody-forming cell clones in the absence of antigen or mitogen; substantially higher cloning efficiency at optimal antigen or mitogen concentration; a slightly lower antigen concentration optimum; and an abnormally high proportion of large cells among the s-IgM+, s-IgD- lymphocyte subset. All these differences argue for a heightened excitability of the NZB B cell to triggering stimuli. Although there was a small-to-moderate increase in the proportion of s-IgM+, s-IgD- b cells, the differences in μ:δ ratio were less than those previously reported. Finally, only a minor and barely significant resistance to tolerance induction in vitro was observed. The results suggest that the heightened B-cell excitability is only one factor in the etiology of autoimmune disease.

Original languageEnglish (US)
Pages (from-to)385-399
Number of pages15
JournalCellular Immunology
Volume63
Issue number2
DOIs
StatePublished - Sep 15 1981
Externally publishedYes

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B-Cell Antigen Receptors
Immunoglobulin Isotypes
New Zealand
B-Lymphocytes
Immunoglobulin D
Mitogens
Antigens
Autoimmune Diseases
Immunoglobulin M
Organism Cloning
Specific Pathogen-Free Organisms
Lymphocyte Subsets
Clone Cells
Fluorescence
Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Surface immunoglobulin isotype, activation, and tolerance susceptibility of B lymphocytes of New Zealand black mice. / Pike, B. L.; Layton, J. E.; Rose, N. R.; Nossal, G. J V.

In: Cellular Immunology, Vol. 63, No. 2, 15.09.1981, p. 385-399.

Research output: Contribution to journalArticle

Pike, B. L. ; Layton, J. E. ; Rose, N. R. ; Nossal, G. J V. / Surface immunoglobulin isotype, activation, and tolerance susceptibility of B lymphocytes of New Zealand black mice. In: Cellular Immunology. 1981 ; Vol. 63, No. 2. pp. 385-399.
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