Surface immobilization of MEPE peptide onto HA/β-TCP ceramic particles enhances bone regeneration and remodeling

Bodhraj Acharya, So Young Chun, Shin Yoon Kim, Cheil Moon, Hong In Shin, Eui Kyun Park

Research output: Contribution to journalArticle

Abstract

Calcium phosphate ceramics have been widely used as scaffolds for bone regeneration. Here, to improve the osteogenic potential of hydroxyapatite/ β-tricalcium phosphate (HA/β-TCP) and to apply the bioactive peptide in situ, matrix extracellular phosphoglycoprotein (MEPE) peptide, which has been shown to stimulate osteoblast differentiation, was covalently and directionally immobilized on HA/β-TCP particles. The free-hydroxyl groups on the surface of the HA/β-TCP particles were sequentially conjugated with APTES, PEG-(SS) 2, and the synthetic MEPE peptide. Using FTIR and XPS, immobilization of the MEPE peptide on the HA/β-TCP was confirmed. Implantation of the MEPE peptide-immobilized HA/β-TCP into calvarial defect and subsequent analyses using a micro CT and histology showed significant bone regeneration and increased bone area (9.89-fold) as compared to that of unmodified HA/β-TCP. Moreover, tartrate-resistant acid phosphatase-positive osteoclasts were observed in regenerated bone by the MEPE peptide-immobilized HA/β-TCP, indicating that the bones newly formed by the MEPE peptide-immobilized HA/β-TCP are actively remodeled by osteoclasts. Therefore, our data demonstrate that MEPE peptide immobilization onto the HA/β-TCP surface stimulates bone regeneration associated with physiological bone remodeling.

Original languageEnglish (US)
Pages (from-to)841-849
Number of pages9
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume100 B
Issue number3
DOIs
StatePublished - Apr 2012
Externally publishedYes

Keywords

  • bone regeneration
  • hydroxyapatite
  • MEPE and bone marrow stem cell
  • surface modification

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

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