Surface imaging microscope

Eric W. Rogala, Isaac N. Bankman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The three-dimensional shapes of microscopic objects are becoming increasingly important for battlespace CBRNE sensing. Potential applications of microscopic 3D shape observations include characterization of biological weapon particles and manufacturing of micromechanical components. Aerosol signatures of stand-off lidar systems, using elastic backscatter or polarization, are dictated by the aerosol particle shapes and sizes that must be well characterized in the lab. A low-cost, fast instrument for 3D surface shape microscopy will be a valuable point sensor for biological particle sensing applications. Both the cost and imaging durations of traditional techniques such as confocal microscopes, atomic force microscopes, and electron scanning microscopes are too high. We investigated the feasibility of a low-cost, fast interferometric technique for imaging the 3D surface shape of microscopic objects at frame rates limited only by the camera in the system. The system operates at two laser wavelengths producing two fringe images collected simultaneously by a digital camera, and a specialized algorithm we developed reconstructs the surface map of the microscopic object. The current implementation assembled to test the concept and develop the new 3D reconstruction algorithm has 0.25 micron resolution in the x and y directions, and about 0.1 micron accuracy in the z direction, as tested on a microscopic glass test object manufactured with etching techniques. We describe the interferometric instrument, present the reconstruction algorithm, and discuss further development.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Volume6954
DOIs
StatePublished - 2008
EventChemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing IX - Orlando, FL, United States
Duration: Mar 18 2008Mar 20 2008

Other

OtherChemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing IX
CountryUnited States
CityOrlando, FL
Period3/18/083/20/08

Fingerprint

Microscopes
microscopes
Imaging techniques
Particles (particulate matter)
Aerosols
Biological weapons
Costs
Digital cameras
Optical radar
aerosols
biological weapons
two-wavelength lasers
Etching
elastic systems
Microscopic examination
Cameras
Polarization
digital cameras
Scanning
Glass

Keywords

  • Aerosols
  • Biological weapons
  • Characterization
  • Detection
  • Fringes
  • Interferometry
  • Microscopy
  • Particles
  • Profilometry
  • Surface map

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Rogala, E. W., & Bankman, I. N. (2008). Surface imaging microscope. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 6954). [69540Z] https://doi.org/10.1117/12.764959

Surface imaging microscope. / Rogala, Eric W.; Bankman, Isaac N.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 6954 2008. 69540Z.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Rogala, EW & Bankman, IN 2008, Surface imaging microscope. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 6954, 69540Z, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing IX, Orlando, FL, United States, 3/18/08. https://doi.org/10.1117/12.764959
Rogala EW, Bankman IN. Surface imaging microscope. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 6954. 2008. 69540Z https://doi.org/10.1117/12.764959
Rogala, Eric W. ; Bankman, Isaac N. / Surface imaging microscope. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 6954 2008.
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