Surface-enhanced Raman spectroscopy on a surface plasmon resonance biosensor platform for gene diagnostics

W. Yuan; H. P. Ho; Y. K. Suen; S. K. Kong; Chinlon Lin; Paras N. Prasad; J. Li; Daniel H.C. Ong

doi:10.1117/12.762286

Surface-enhanced Raman spectroscopy on a surface plasmon resonance biosensor platform for gene diagnostics

W. Yuan, H. P. Ho, Y. K. Suen, S. K. Kong, Chinlon Lin, Paras N. Prasad, J. Li, Daniel H.C. Ong

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We propose to integrate the surface-enhanced Raman spectroscopy (SERS) detection capability with a surface plasmon resonance (SPR) biosensor platform. As a demonstration setup, the experimental scheme is built from a Total Internal Reflection Fluorescence (TIRF) microscope. The sample surface is a gold-coated plasmonic crystal substrate. Two oligonucleotide (ODN) probes that have been labeled with two different Raman active dyes are used to achieve a sandwich assay of target ODNs or polynucleotide. Upon complementary hybridizations between the target and probe ODNs, the target can be identified by detecting the narrow-band spectroscopic fingerprints of the Raman tags. This concept has high potential for achieving multiplexed detection of ODN targets because a very large number of probes can be incorporated to the plasmonic crystal substrate, which may find applications in gene based diseases diagnostics. We also explored the detection of single molecules and achieved some preliminary results.

Original language	English (US)
Title of host publication	Plasmonics in Biology and Medicine V
DOIs	https://doi.org/10.1117/12.762286
State	Published - 2008
Externally published	Yes
Event	Plasmonics in Biology and Medicine V - San Jose, CA, United States Duration: Jan 21 2008 → Jan 22 2008

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6869
ISSN (Print)	1605-7422

Other

Other	Plasmonics in Biology and Medicine V
Country/Territory	United States
City	San Jose, CA
Period	1/21/08 → 1/22/08

Keywords

DNA
Surface plasmon resonance
Surface-enhanced Raman

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.762286

Cite this

Yuan, W., Ho, H. P., Suen, Y. K., Kong, S. K., Lin, C., Prasad, P. N., Li, J., & Ong, D. H. C. (2008). Surface-enhanced Raman spectroscopy on a surface plasmon resonance biosensor platform for gene diagnostics. In Plasmonics in Biology and Medicine V Article 686902 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6869). https://doi.org/10.1117/12.762286

Yuan, W, Ho, HP, Suen, YK, Kong, SK, Lin, C, Prasad, PN, Li, J & Ong, DHC 2008, Surface-enhanced Raman spectroscopy on a surface plasmon resonance biosensor platform for gene diagnostics. in Plasmonics in Biology and Medicine V., 686902, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6869, Plasmonics in Biology and Medicine V, San Jose, CA, United States, 1/21/08. https://doi.org/10.1117/12.762286

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title = "Surface-enhanced Raman spectroscopy on a surface plasmon resonance biosensor platform for gene diagnostics",

abstract = "We propose to integrate the surface-enhanced Raman spectroscopy (SERS) detection capability with a surface plasmon resonance (SPR) biosensor platform. As a demonstration setup, the experimental scheme is built from a Total Internal Reflection Fluorescence (TIRF) microscope. The sample surface is a gold-coated plasmonic crystal substrate. Two oligonucleotide (ODN) probes that have been labeled with two different Raman active dyes are used to achieve a sandwich assay of target ODNs or polynucleotide. Upon complementary hybridizations between the target and probe ODNs, the target can be identified by detecting the narrow-band spectroscopic fingerprints of the Raman tags. This concept has high potential for achieving multiplexed detection of ODN targets because a very large number of probes can be incorporated to the plasmonic crystal substrate, which may find applications in gene based diseases diagnostics. We also explored the detection of single molecules and achieved some preliminary results.",

keywords = "DNA, Surface plasmon resonance, Surface-enhanced Raman",

author = "W. Yuan and Ho, {H. P.} and Suen, {Y. K.} and Kong, {S. K.} and Chinlon Lin and Prasad, {Paras N.} and J. Li and Ong, {Daniel H.C.}",

year = "2008",

doi = "10.1117/12.762286",

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series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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AU - Yuan, W.

AU - Ho, H. P.

AU - Suen, Y. K.

AU - Kong, S. K.

AU - Lin, Chinlon

AU - Prasad, Paras N.

AU - Li, J.

AU - Ong, Daniel H.C.

PY - 2008

Y1 - 2008

N2 - We propose to integrate the surface-enhanced Raman spectroscopy (SERS) detection capability with a surface plasmon resonance (SPR) biosensor platform. As a demonstration setup, the experimental scheme is built from a Total Internal Reflection Fluorescence (TIRF) microscope. The sample surface is a gold-coated plasmonic crystal substrate. Two oligonucleotide (ODN) probes that have been labeled with two different Raman active dyes are used to achieve a sandwich assay of target ODNs or polynucleotide. Upon complementary hybridizations between the target and probe ODNs, the target can be identified by detecting the narrow-band spectroscopic fingerprints of the Raman tags. This concept has high potential for achieving multiplexed detection of ODN targets because a very large number of probes can be incorporated to the plasmonic crystal substrate, which may find applications in gene based diseases diagnostics. We also explored the detection of single molecules and achieved some preliminary results.

AB - We propose to integrate the surface-enhanced Raman spectroscopy (SERS) detection capability with a surface plasmon resonance (SPR) biosensor platform. As a demonstration setup, the experimental scheme is built from a Total Internal Reflection Fluorescence (TIRF) microscope. The sample surface is a gold-coated plasmonic crystal substrate. Two oligonucleotide (ODN) probes that have been labeled with two different Raman active dyes are used to achieve a sandwich assay of target ODNs or polynucleotide. Upon complementary hybridizations between the target and probe ODNs, the target can be identified by detecting the narrow-band spectroscopic fingerprints of the Raman tags. This concept has high potential for achieving multiplexed detection of ODN targets because a very large number of probes can be incorporated to the plasmonic crystal substrate, which may find applications in gene based diseases diagnostics. We also explored the detection of single molecules and achieved some preliminary results.

KW - DNA

KW - Surface plasmon resonance

KW - Surface-enhanced Raman

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T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Plasmonics in Biology and Medicine V

T2 - Plasmonics in Biology and Medicine V

Y2 - 21 January 2008 through 22 January 2008

ER -

Surface-enhanced Raman spectroscopy on a surface plasmon resonance biosensor platform for gene diagnostics

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