TY - JOUR
T1 - Surface-directed DNA condensation in the absence of soluble multivalent cations
AU - Fang, Ye
AU - Hoh, Jan H.
N1 - Funding Information:
We thank Dr Roger Reeves for providing the plasmid used here. This work was supported by NIH grant HGO1518.
PY - 1998/1/15
Y1 - 1998/1/15
N2 - Multivalent cations are known to condense DNA into higher ordered structures, including toroids and rods. Here we report that solid supports treated with monovalent or multivalent cationic silanes, followed by removal of soluble molecules, can condense DNA. The mechanism of this surface-directed condensation depends on surface-mobile silanes, which are apparently recruited to the condensation site. The yield and species of DNA aggregates can be controlled by selecting the type of functional groups on surfaces, DNA and salt concentrations. For plasmid DNA, the toroidal form can represent > 70% of adsorbed structures.
AB - Multivalent cations are known to condense DNA into higher ordered structures, including toroids and rods. Here we report that solid supports treated with monovalent or multivalent cationic silanes, followed by removal of soluble molecules, can condense DNA. The mechanism of this surface-directed condensation depends on surface-mobile silanes, which are apparently recruited to the condensation site. The yield and species of DNA aggregates can be controlled by selecting the type of functional groups on surfaces, DNA and salt concentrations. For plasmid DNA, the toroidal form can represent > 70% of adsorbed structures.
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U2 - 10.1093/nar/26.2.588
DO - 10.1093/nar/26.2.588
M3 - Article
C2 - 9421520
AN - SCOPUS:0032518149
SN - 0305-1048
VL - 26
SP - 588
EP - 593
JO - Nucleic acids research
JF - Nucleic acids research
IS - 2
ER -