TY - JOUR
T1 - Surface cAMP receptors mediate multiple responses during development in Dictyostelium
T2 - Evidenced by antisense mutagenesis
AU - Sun, T. J.
AU - Van Haastert, P. J.M.
AU - Devreotes, P. N.
PY - 1990
Y1 - 1990
N2 - Cell surface cAMP receptors (cARs) have been implicated in multiple aspects of development in Dictyostelium. Antisense mutagenesis has recently provided strong evidence that cARs are necessary for aggregation (Klein et al., 1988. Science (Wash. DC). 241:1467-1472). We show here that the expression of cAR1 antisense mRNA which prevents the appearance of cAR1 antigen also prevents the expression of cAMP-binding activity and blocks multiple cAMP-mediated responses. Chemotactic sensitivity of cAMP was lost as were stimulus-induced cAMP and cGMP production. Furthermore, the expression of developmentally regulated marker genes, dependent on repeated cAMP stimulation, was altered. As a result, the developmental program was severely impaired; most of the cells failed to aggregate and undergo further differentiation.
AB - Cell surface cAMP receptors (cARs) have been implicated in multiple aspects of development in Dictyostelium. Antisense mutagenesis has recently provided strong evidence that cARs are necessary for aggregation (Klein et al., 1988. Science (Wash. DC). 241:1467-1472). We show here that the expression of cAR1 antisense mRNA which prevents the appearance of cAR1 antigen also prevents the expression of cAMP-binding activity and blocks multiple cAMP-mediated responses. Chemotactic sensitivity of cAMP was lost as were stimulus-induced cAMP and cGMP production. Furthermore, the expression of developmentally regulated marker genes, dependent on repeated cAMP stimulation, was altered. As a result, the developmental program was severely impaired; most of the cells failed to aggregate and undergo further differentiation.
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U2 - 10.1083/jcb.110.5.1549
DO - 10.1083/jcb.110.5.1549
M3 - Article
C2 - 1692327
AN - SCOPUS:0025294914
SN - 0021-9525
VL - 110
SP - 1549
EP - 1554
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 5
ER -