Suprabasin Is Hypomethylated and Associated with Metastasis in Salivary Adenoid Cystic Carcinoma

Chunbo Shao, Marietta Tan, Justin A. Bishop, Jia Liu, Weiliang Bai, Daria Gaykalova, Takenori Ogawa, Ami R. Vikani, Yuri Agrawal, Ryan J. Li, Myoung Sook Kim, William H. Westra, David Sidransky, Joseph A. Califano, Patrick K. Ha

Research output: Contribution to journalArticle

Abstract

Background: Salivary gland adenoid cystic carcinoma (ACC) is a rare cancer, accounting for only 1% of all head and neck malignancies. ACC is well known for perineural invasion and distant metastasis, but its underlying molecular mechanisms of carcinogenesis are still unclear. Principal Findings: Here, we show that a novel oncogenic candidate, suprabasin (SBSN), plays important roles in maintaining the anchorage-independent and anchorage-dependent cell proliferation in ACC by using SBSN shRNA stably transfected ACC cell line clones. SBSN is also important in maintaining the invasive/metastatic capability in ACC by Matrigel invasion assay. More interestingly, SBSN transcription is significantly upregulated by DNA demethylation induced by 5-aza-2′-deoxycytidine plus trichostatin A treatment and the DNA methylation levels of the SBSN CpG island located in the second intron were validated to be significantly hypomethylated in primary ACC samples versus normal salivary gland tissues. Conclusions/Significance: Taken together, these results support SBSN as novel oncogene candidate in ACC, and the methylation changes could be a promising biomarker for ACC.

Original languageEnglish (US)
Article numbere48582
JournalPLoS One
Volume7
Issue number11
DOIs
StatePublished - Nov 7 2012

Fingerprint

decitabine
trichostatin A
Adenoid Cystic Carcinoma
CpG Islands
Methylation
Cell proliferation
Biomarkers
Transcription
metastasis
Introns
Small Interfering RNA
carcinoma
Assays
Cells
Tissue
Neoplasm Metastasis
DNA
salivary glands
Salivary Glands
matrigel

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Suprabasin Is Hypomethylated and Associated with Metastasis in Salivary Adenoid Cystic Carcinoma. / Shao, Chunbo; Tan, Marietta; Bishop, Justin A.; Liu, Jia; Bai, Weiliang; Gaykalova, Daria; Ogawa, Takenori; Vikani, Ami R.; Agrawal, Yuri; Li, Ryan J.; Kim, Myoung Sook; Westra, William H.; Sidransky, David; Califano, Joseph A.; Ha, Patrick K.

In: PLoS One, Vol. 7, No. 11, e48582, 07.11.2012.

Research output: Contribution to journalArticle

Shao, C, Tan, M, Bishop, JA, Liu, J, Bai, W, Gaykalova, D, Ogawa, T, Vikani, AR, Agrawal, Y, Li, RJ, Kim, MS, Westra, WH, Sidransky, D, Califano, JA & Ha, PK 2012, 'Suprabasin Is Hypomethylated and Associated with Metastasis in Salivary Adenoid Cystic Carcinoma', PLoS One, vol. 7, no. 11, e48582. https://doi.org/10.1371/journal.pone.0048582
Shao, Chunbo ; Tan, Marietta ; Bishop, Justin A. ; Liu, Jia ; Bai, Weiliang ; Gaykalova, Daria ; Ogawa, Takenori ; Vikani, Ami R. ; Agrawal, Yuri ; Li, Ryan J. ; Kim, Myoung Sook ; Westra, William H. ; Sidransky, David ; Califano, Joseph A. ; Ha, Patrick K. / Suprabasin Is Hypomethylated and Associated with Metastasis in Salivary Adenoid Cystic Carcinoma. In: PLoS One. 2012 ; Vol. 7, No. 11.
@article{e23d2ab718b0401f89e3c5505f7b3922,
title = "Suprabasin Is Hypomethylated and Associated with Metastasis in Salivary Adenoid Cystic Carcinoma",
abstract = "Background: Salivary gland adenoid cystic carcinoma (ACC) is a rare cancer, accounting for only 1{\%} of all head and neck malignancies. ACC is well known for perineural invasion and distant metastasis, but its underlying molecular mechanisms of carcinogenesis are still unclear. Principal Findings: Here, we show that a novel oncogenic candidate, suprabasin (SBSN), plays important roles in maintaining the anchorage-independent and anchorage-dependent cell proliferation in ACC by using SBSN shRNA stably transfected ACC cell line clones. SBSN is also important in maintaining the invasive/metastatic capability in ACC by Matrigel invasion assay. More interestingly, SBSN transcription is significantly upregulated by DNA demethylation induced by 5-aza-2′-deoxycytidine plus trichostatin A treatment and the DNA methylation levels of the SBSN CpG island located in the second intron were validated to be significantly hypomethylated in primary ACC samples versus normal salivary gland tissues. Conclusions/Significance: Taken together, these results support SBSN as novel oncogene candidate in ACC, and the methylation changes could be a promising biomarker for ACC.",
author = "Chunbo Shao and Marietta Tan and Bishop, {Justin A.} and Jia Liu and Weiliang Bai and Daria Gaykalova and Takenori Ogawa and Vikani, {Ami R.} and Yuri Agrawal and Li, {Ryan J.} and Kim, {Myoung Sook} and Westra, {William H.} and David Sidransky and Califano, {Joseph A.} and Ha, {Patrick K.}",
year = "2012",
month = "11",
day = "7",
doi = "10.1371/journal.pone.0048582",
language = "English (US)",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

TY - JOUR

T1 - Suprabasin Is Hypomethylated and Associated with Metastasis in Salivary Adenoid Cystic Carcinoma

AU - Shao, Chunbo

AU - Tan, Marietta

AU - Bishop, Justin A.

AU - Liu, Jia

AU - Bai, Weiliang

AU - Gaykalova, Daria

AU - Ogawa, Takenori

AU - Vikani, Ami R.

AU - Agrawal, Yuri

AU - Li, Ryan J.

AU - Kim, Myoung Sook

AU - Westra, William H.

AU - Sidransky, David

AU - Califano, Joseph A.

AU - Ha, Patrick K.

PY - 2012/11/7

Y1 - 2012/11/7

N2 - Background: Salivary gland adenoid cystic carcinoma (ACC) is a rare cancer, accounting for only 1% of all head and neck malignancies. ACC is well known for perineural invasion and distant metastasis, but its underlying molecular mechanisms of carcinogenesis are still unclear. Principal Findings: Here, we show that a novel oncogenic candidate, suprabasin (SBSN), plays important roles in maintaining the anchorage-independent and anchorage-dependent cell proliferation in ACC by using SBSN shRNA stably transfected ACC cell line clones. SBSN is also important in maintaining the invasive/metastatic capability in ACC by Matrigel invasion assay. More interestingly, SBSN transcription is significantly upregulated by DNA demethylation induced by 5-aza-2′-deoxycytidine plus trichostatin A treatment and the DNA methylation levels of the SBSN CpG island located in the second intron were validated to be significantly hypomethylated in primary ACC samples versus normal salivary gland tissues. Conclusions/Significance: Taken together, these results support SBSN as novel oncogene candidate in ACC, and the methylation changes could be a promising biomarker for ACC.

AB - Background: Salivary gland adenoid cystic carcinoma (ACC) is a rare cancer, accounting for only 1% of all head and neck malignancies. ACC is well known for perineural invasion and distant metastasis, but its underlying molecular mechanisms of carcinogenesis are still unclear. Principal Findings: Here, we show that a novel oncogenic candidate, suprabasin (SBSN), plays important roles in maintaining the anchorage-independent and anchorage-dependent cell proliferation in ACC by using SBSN shRNA stably transfected ACC cell line clones. SBSN is also important in maintaining the invasive/metastatic capability in ACC by Matrigel invasion assay. More interestingly, SBSN transcription is significantly upregulated by DNA demethylation induced by 5-aza-2′-deoxycytidine plus trichostatin A treatment and the DNA methylation levels of the SBSN CpG island located in the second intron were validated to be significantly hypomethylated in primary ACC samples versus normal salivary gland tissues. Conclusions/Significance: Taken together, these results support SBSN as novel oncogene candidate in ACC, and the methylation changes could be a promising biomarker for ACC.

UR - http://www.scopus.com/inward/record.url?scp=84868686494&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84868686494&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0048582

DO - 10.1371/journal.pone.0048582

M3 - Article

C2 - 23144906

AN - SCOPUS:84868686494

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 11

M1 - e48582

ER -