Suppression of prostate cancer invasive potential and matrix metalloproteinase activity by E-cadherin transfection

Jun Luo, David M. Lubaroff, Mary J C Hendrix

Research output: Contribution to journalArticle

Abstract

Our previous studies have demonstrated the heterogeneous expression of E-cadherin in a Dunning rat prostate tumor model. From this model, cloned E- cadherin-negative cells exhibited enhanced invasive and metastatic potential when compared with E-cadherin-positive cells. In this report, we examined the invasion suppressor function of E-cadherin in these prostate tumor cell clones. The E-cadherin gene was stably transfected into E-cadherin-negative Dunning clones. E-cadherin transfection resulted in the up-regulation of the three major catenins (α-, β-, and γ-catenin) and enhanced Ca2+-dependent cellular cohesiveness. Morphological analyses of E-cadherin transfectants revealed a reversion from a fibroblastic, motile phenotype to a more stationary epithelial phenotype. Matrix metalloproteinase 2, an important marker associated with invasive and metastatic potential, was reduced in all six stable transfected lines. A concomitant decrease in cellular invasiveness was observed, as assessed in vitro by the ability of the transfected cells to invade biological matrices. These results lend further support to the hypothesis that in this experimental system, E-cadherin plays a central role in reducing the cellular invasiveness of prostatic adenocarcinoma, due in part to the down-regulation of matrix metalloproteinase 2 activity. Moreover, the data shed additional light on the possible mechanisms involved in E- cadherin-dependent modulation of invasion.

Original languageEnglish (US)
Pages (from-to)3552-3556
Number of pages5
JournalCancer Research
Volume59
Issue number15
StatePublished - Aug 1 1999
Externally publishedYes

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Cadherins
Matrix Metalloproteinases
Transfection
Prostatic Neoplasms
Catenins
Matrix Metalloproteinase 2
Prostate
Clone Cells
Phenotype
Neoplasms
Adenocarcinoma
Up-Regulation
Down-Regulation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Suppression of prostate cancer invasive potential and matrix metalloproteinase activity by E-cadherin transfection. / Luo, Jun; Lubaroff, David M.; Hendrix, Mary J C.

In: Cancer Research, Vol. 59, No. 15, 01.08.1999, p. 3552-3556.

Research output: Contribution to journalArticle

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