Suppression of linear side products by macromolecular crowding in nonribosomal enterobactin biosynthesis

Zu Feng Guo, Ming Jiang, Suilan Zheng, Zhihong Guo

Research output: Contribution to journalArticle

Abstract

Nonribosomal enterobactin synthetase of Escherichia coli was found to prematurely release a large amount of linear precursors in an in vitro reconstitution. However, these side products are suppressed to negligible levels by polymeric cosolvents that create macromolecular crowding, a prominent feature of the intracellular environment. These findings show that macromolecular crowding is essential to normal functioning of the nonribosomal peptide synthetase and suggest that it may be crucial to btotechnological utilization of similar enzyme systems.

Original languageEnglish (US)
Pages (from-to)649-652
Number of pages4
JournalOrganic Letters
Volume10
Issue number4
DOIs
StatePublished - Feb 21 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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