Suppression of linear side products by macromolecular crowding in nonribosomal enterobactin biosynthesis

Zu Feng Guo; Ming Jiang; Suilan Zheng; Zhihong Guo

doi:10.1021/ol7030153

Suppression of linear side products by macromolecular crowding in nonribosomal enterobactin biosynthesis

Zu Feng Guo, Ming Jiang, Suilan Zheng, Zhihong Guo

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Nonribosomal enterobactin synthetase of Escherichia coli was found to prematurely release a large amount of linear precursors in an in vitro reconstitution. However, these side products are suppressed to negligible levels by polymeric cosolvents that create macromolecular crowding, a prominent feature of the intracellular environment. These findings show that macromolecular crowding is essential to normal functioning of the nonribosomal peptide synthetase and suggest that it may be crucial to btotechnological utilization of similar enzyme systems.

Original language	English (US)
Pages (from-to)	649-652
Number of pages	4
Journal	Organic Letters
Volume	10
Issue number	4
DOIs	https://doi.org/10.1021/ol7030153
State	Published - Feb 21 2008
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1021/ol7030153

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abstract = "Nonribosomal enterobactin synthetase of Escherichia coli was found to prematurely release a large amount of linear precursors in an in vitro reconstitution. However, these side products are suppressed to negligible levels by polymeric cosolvents that create macromolecular crowding, a prominent feature of the intracellular environment. These findings show that macromolecular crowding is essential to normal functioning of the nonribosomal peptide synthetase and suggest that it may be crucial to btotechnological utilization of similar enzyme systems.",

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AU - Jiang, Ming

AU - Zheng, Suilan

AU - Guo, Zhihong

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AB - Nonribosomal enterobactin synthetase of Escherichia coli was found to prematurely release a large amount of linear precursors in an in vitro reconstitution. However, these side products are suppressed to negligible levels by polymeric cosolvents that create macromolecular crowding, a prominent feature of the intracellular environment. These findings show that macromolecular crowding is essential to normal functioning of the nonribosomal peptide synthetase and suggest that it may be crucial to btotechnological utilization of similar enzyme systems.

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Suppression of linear side products by macromolecular crowding in nonribosomal enterobactin biosynthesis

Abstract

ASJC Scopus subject areas

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