Suppression and potentiation of mouse hematopoietic progenitor cell proliferation by ouabain

J. L. Spivak, J. Misiti, R. Stuart, S. J. Sharkis, L. L. Sensenbrenner

Research output: Contribution to journalArticlepeer-review

Abstract

Clonal assays for CFU-S, CFU-C, BFU-E, and CFU-E were employed to evaluate the effect of ouabain on the proliferation of mouse hematopoietic progenitor cells. Preincubation of bone marrow cells with ouabain at concentrations of 10-6-10-12 M suppressed the proliferation of CFU-S as measured by the spleen colony assay. At 10-9 M ouabain, spleen colony formation was inhibited by more than 95%. When included in soft agar cultures of bone marrow cells, ouabain suppressed the proliferation of CFU-C in a complex fashion. At 10-4 M ouabain, colony formation was suppressed by 70%, while at 10-6 M ouabain, CFU-C proliferation was normal. At 10-9 M ouabain, however, the number of colonies formed was only 70% of normal, and complete recovery was not obtained at 10-12 M ouabain. The number of colonies formed by BFU-E and CFU-E in plasma clot cultures was also diminished in the presence of 10-4 M ouabain. In contrast to its effect on CFU-S and CFU-C, however, 10-9 M ouabain enhanced BFU-E colony formation by 200% and CFU-E colony formation by 35%; at 10-12 M ouabain, CFU-E proliferation was unaffected by the drug. These data indicate that mouse CFU-S, CFU-C, BFU-E, and CFU-E require a functional membrane-bound Na+K+ATPase for proliferation; that these progenitor cells differ in their response to ouabain and finally, that ouabain at concentrations below that (10-6 M) considered necessary to inhibit membrane-bound Na+K+ATPase can influence the proliferation of mouse hematopoietic progenitor cells.

Original languageEnglish (US)
Pages (from-to)315-317
Number of pages3
JournalBlood
Volume56
Issue number2
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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