Suppression and potentiation of mouse hematopoietic progenitor cell proliferation by ouabain

Jerry L Spivak, J. Misiti, R. Stuart, S. J. Sharkis, L. L. Sensenbrenner

Research output: Contribution to journalArticle

Abstract

Clonal assays for CFU-S, CFU-C, BFU-E, and CFU-E were employed to evaluate the effect of ouabain on the proliferation of mouse hematopoietic progenitor cells. Preincubation of bone marrow cells with ouabain at concentrations of 10 -6-10 -12 M suppressed the proliferation of CFU-S as measured by the spleen colony assay. At 10 -9 M ouabain, spleen colony formation was inhibited by more than 95%. When included in soft agar cultures of bone marrow cells, ouabain suppressed the proliferation of CFU-C in a complex fashion. At 10 -4 M ouabain, colony formation was suppressed by 70%, while at 10 -6 M ouabain, CFU-C proliferation was normal. At 10 -9 M ouabain, however, the number of colonies formed was only 70% of normal, and complete recovery was not obtained at 10 -12 M ouabain. The number of colonies formed by BFU-E and CFU-E in plasma clot cultures was also diminished in the presence of 10 -4 M ouabain. In contrast to its effect on CFU-S and CFU-C, however, 10 -9 M ouabain enhanced BFU-E colony formation by 200% and CFU-E colony formation by 35%; at 10 -12 M ouabain, CFU-E proliferation was unaffected by the drug. These data indicate that mouse CFU-S, CFU-C, BFU-E, and CFU-E require a functional membrane-bound Na +K +ATPase for proliferation; that these progenitor cells differ in their response to ouabain and finally, that ouabain at concentrations below that (10 -6 M) considered necessary to inhibit membrane-bound Na +K +ATPase can influence the proliferation of mouse hematopoietic progenitor cells.

Original languageEnglish (US)
Pages (from-to)315-317
Number of pages3
JournalBlood
Volume56
Issue number2
StatePublished - 1980

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Cell proliferation
Ouabain
Hematopoietic Stem Cells
Erythroid Precursor Cells
Cell Proliferation
Bone Marrow Cells
Adenosine Triphosphatases
Assays
Bone
Spleen
Cells
Membranes
Cell culture
Agar
Stem Cells

ASJC Scopus subject areas

  • Hematology

Cite this

Spivak, J. L., Misiti, J., Stuart, R., Sharkis, S. J., & Sensenbrenner, L. L. (1980). Suppression and potentiation of mouse hematopoietic progenitor cell proliferation by ouabain. Blood, 56(2), 315-317.

Suppression and potentiation of mouse hematopoietic progenitor cell proliferation by ouabain. / Spivak, Jerry L; Misiti, J.; Stuart, R.; Sharkis, S. J.; Sensenbrenner, L. L.

In: Blood, Vol. 56, No. 2, 1980, p. 315-317.

Research output: Contribution to journalArticle

Spivak, JL, Misiti, J, Stuart, R, Sharkis, SJ & Sensenbrenner, LL 1980, 'Suppression and potentiation of mouse hematopoietic progenitor cell proliferation by ouabain', Blood, vol. 56, no. 2, pp. 315-317.
Spivak JL, Misiti J, Stuart R, Sharkis SJ, Sensenbrenner LL. Suppression and potentiation of mouse hematopoietic progenitor cell proliferation by ouabain. Blood. 1980;56(2):315-317.
Spivak, Jerry L ; Misiti, J. ; Stuart, R. ; Sharkis, S. J. ; Sensenbrenner, L. L. / Suppression and potentiation of mouse hematopoietic progenitor cell proliferation by ouabain. In: Blood. 1980 ; Vol. 56, No. 2. pp. 315-317.
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