Superresolution Modeling of Calcium Release in the Heart

Mark A. Walker, George S B Williams, Tobias Kohl, Stephan E. Lehnart, M. Saleet Jafri, Joseph L. Greenstein, W. J. Lederer, Raimond Winslow

Research output: Contribution to journalArticle

Abstract

Stable calcium-induced calcium release (CICR) is critical for maintaining normal cellular contraction during cardiac excitation-contraction coupling. The fundamental element of CICR in the heart is the calcium (Ca2+) spark, which arises from a cluster of ryanodine receptors (RyR). Opening of these RyR clusters is triggered to produce a local, regenerative release of Ca2+ from the sarcoplasmic reticulum (SR). The Ca2+ leak out of the SR is an important process for cellular Ca2+ management, and it is critically influenced by spark fidelity, i.e., the probability that a spontaneous RyR opening triggers a Ca2+ spark. Here, we present a detailed, three-dimensional model of a cardiac Ca2+ release unit that incorporates diffusion, intracellular buffering systems, and stochastically gated ion channels. The model exhibits realistic Ca2+ sparks and robust Ca2+ spark termination across a wide range of geometries and conditions. Furthermore, the model captures the details of Ca2+ spark and nonspark-based SR Ca2+ leak, and it produces normal excitation-contraction coupling gain. We show that SR luminal Ca2+-dependent regulation of the RyR is not critical for spark termination, but it can explain the exponential rise in the SR Ca2+ leak-load relationship demonstrated in previous experimental work. Perturbations to subspace dimensions, which have been observed in experimental models of disease, strongly alter Ca2+ spark dynamics. In addition, we find that the structure of RyR clusters also influences Ca2+ release properties due to variations in inter-RyR coupling via local subspace Ca2+ concentration ([Ca2+]ss). These results are illustrated for RyR clusters based on super-resolution stimulated emission depletion microscopy. Finally, we present a believed-novel approach by which the spark fidelity of a RyR cluster can be predicted from structural information of the cluster using the maximum eigenvalue of its adjacency matrix. These results provide critical insights into CICR dynamics in heart, under normal and pathological conditions.

Original languageEnglish (US)
Pages (from-to)3018-3029
Number of pages12
JournalBiophysical Journal
Volume107
Issue number12
DOIs
StatePublished - 2014

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Ryanodine Receptor Calcium Release Channel
Sarcoplasmic Reticulum
Calcium
Excitation Contraction Coupling
Calcium Signaling
Ion Channels
Microscopy
Theoretical Models

ASJC Scopus subject areas

  • Biophysics

Cite this

Walker, M. A., Williams, G. S. B., Kohl, T., Lehnart, S. E., Jafri, M. S., Greenstein, J. L., ... Winslow, R. (2014). Superresolution Modeling of Calcium Release in the Heart. Biophysical Journal, 107(12), 3018-3029. https://doi.org/10.1016/j.bpj.2014.11.003

Superresolution Modeling of Calcium Release in the Heart. / Walker, Mark A.; Williams, George S B; Kohl, Tobias; Lehnart, Stephan E.; Jafri, M. Saleet; Greenstein, Joseph L.; Lederer, W. J.; Winslow, Raimond.

In: Biophysical Journal, Vol. 107, No. 12, 2014, p. 3018-3029.

Research output: Contribution to journalArticle

Walker, MA, Williams, GSB, Kohl, T, Lehnart, SE, Jafri, MS, Greenstein, JL, Lederer, WJ & Winslow, R 2014, 'Superresolution Modeling of Calcium Release in the Heart', Biophysical Journal, vol. 107, no. 12, pp. 3018-3029. https://doi.org/10.1016/j.bpj.2014.11.003
Walker MA, Williams GSB, Kohl T, Lehnart SE, Jafri MS, Greenstein JL et al. Superresolution Modeling of Calcium Release in the Heart. Biophysical Journal. 2014;107(12):3018-3029. https://doi.org/10.1016/j.bpj.2014.11.003
Walker, Mark A. ; Williams, George S B ; Kohl, Tobias ; Lehnart, Stephan E. ; Jafri, M. Saleet ; Greenstein, Joseph L. ; Lederer, W. J. ; Winslow, Raimond. / Superresolution Modeling of Calcium Release in the Heart. In: Biophysical Journal. 2014 ; Vol. 107, No. 12. pp. 3018-3029.
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