Superresolution imaging of targeted proteins in fixed and living cells using photoactivatable organic fluorophores

Hsiao Lu D. Lee, Samuel J. Lord, Shigeki Iwanaga, Ke Zhan, Hexin Xie, Jarrod C. Williams, Hui Wang, Grant R. Bowman, Erin D. Goley, Lucy Shapiro, Robert J. Twieg, Jianghong Rao, W. E. Moerner

Research output: Contribution to journalArticlepeer-review

Abstract

Superresolution imaging techniques based on sequential imaging of sparse subsets of single molecules require fluorophores whose emission can be photoactivated or photoswitched. Because typical organic fluorophores can emit significantly more photons than average fluorescent proteins, organic fluorophores have a potential advantage in super-resolution imaging schemes, but targeting to specific cellular proteins must be provided. We report the design and application of HaloTag-based target-specific azido DCDHFs, a class of photoactivatable push-pull fluorogens which produce bright fluorescent labels suitable for single-molecule superresolution imaging in live bacterial and fixed mammalian cells.

Original languageEnglish (US)
Pages (from-to)15099-15101
Number of pages3
JournalJournal of the American Chemical Society
Volume132
Issue number43
DOIs
StatePublished - Nov 3 2010
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'Superresolution imaging of targeted proteins in fixed and living cells using photoactivatable organic fluorophores'. Together they form a unique fingerprint.

Cite this