Superparamagnetic iron oxide labeling of stem cells for MRI tracking and delivery in cardiovascular disease

Dorota A. Kedziorek, Dara L. Kraitchman

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In the mid-1980s, iron oxide nanoparticles were developed as contrast agents for diagnostic imaging. In the last two decades, established methods to label cells with superparamagnetic iron oxides (SPIOs) have been developed to aid in targeted delivery and tracking of stem cell therapies. The surge in cellular therapy clinical trials for cardiovascular applications has seen a similar rise in the number of preclinical animal studies of SPIO-labeled stem cells in an effort to understand the mechanisms of cardiovascular regenerative therapy and stem cell biodistribution. The adoption of a limited number of methods of direct labeling of stem cells with SPIOs is due in large part to the desire to rapidly translate these techniques to clinical trials. In this review, we will outline the most commonly adopted methods for iron oxide labeling of stem cells for cardiovascular applications and describe strategies for magnetic resonance imaging (MRI) of magnetically labeled cells in the heart.

Original languageEnglish (US)
Title of host publicationStem Cells for Myocardial Regeneration
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages171-183
Number of pages13
ISBN (Print)9781607617044
DOIs
StatePublished - 2010

Publication series

NameMethods in Molecular Biology
Volume660
ISSN (Print)1064-3745

Keywords

  • Cell imaging
  • Cellular labeling
  • Electroporation
  • Magnetic resonance imaging (MRI)
  • Stem cells
  • Superparamagnetic iron oxide (SPIO)
  • Transfection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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