TY - JOUR
T1 - Superoxide regulation of endothelin-converting enzyme
AU - López-Ongil, Susana
AU - Senchak, Veronica
AU - Saura, Marta
AU - Zaragoza, Carlos
AU - Ames, Michael
AU - Ballermann, Barbara
AU - Rodríguez-Puyol, Manuel
AU - Rodríguez-Puyol, Diego
AU - Lowensteini, Charles J.
PY - 2000/8/25
Y1 - 2000/8/25
N2 - Reactive oxygen species (ROS) act as signaling molecules in the cardiovascular system, regulating cellular proliferation and migration. However, an excess of ROS can damage cells and alter endothelial cell function. We hypothesized that endogenous mechanisms protect the vasculature from excess levels of ROS. We now show that superoxide can inhibit endothelin-converting enzyme activity (ECE) and decrease endothelin-1 synthesis. Superoxide inhibits ECE but hydrogen peroxide and nitric oxide do not. Superoxide inhibits ECE by ejecting zinc from the enzyme, and the addition of exogenous zinc restores enzymatic activity. Superoxide may inhibit other zinc metalloproteinases by a similar mechanism and may thus play an important role in regulating the biology of blood vessels.
AB - Reactive oxygen species (ROS) act as signaling molecules in the cardiovascular system, regulating cellular proliferation and migration. However, an excess of ROS can damage cells and alter endothelial cell function. We hypothesized that endogenous mechanisms protect the vasculature from excess levels of ROS. We now show that superoxide can inhibit endothelin-converting enzyme activity (ECE) and decrease endothelin-1 synthesis. Superoxide inhibits ECE but hydrogen peroxide and nitric oxide do not. Superoxide inhibits ECE by ejecting zinc from the enzyme, and the addition of exogenous zinc restores enzymatic activity. Superoxide may inhibit other zinc metalloproteinases by a similar mechanism and may thus play an important role in regulating the biology of blood vessels.
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U2 - 10.1074/jbc.M000767200
DO - 10.1074/jbc.M000767200
M3 - Article
C2 - 10833511
AN - SCOPUS:0034714381
SN - 0021-9258
VL - 275
SP - 26423
EP - 26427
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 34
ER -