Superoxide regulation of endothelin-converting enzyme

Susana López-Ongil, Veronica Senchak, Marta Saura, Carlos Zaragoza, Michael Ames, Barbara Ballermann, Manuel Rodríguez-Puyol, Diego Rodríguez-Puyol, Charles J. Lowensteini

Research output: Contribution to journalArticle

Abstract

Reactive oxygen species (ROS) act as signaling molecules in the cardiovascular system, regulating cellular proliferation and migration. However, an excess of ROS can damage cells and alter endothelial cell function. We hypothesized that endogenous mechanisms protect the vasculature from excess levels of ROS. We now show that superoxide can inhibit endothelin-converting enzyme activity (ECE) and decrease endothelin-1 synthesis. Superoxide inhibits ECE but hydrogen peroxide and nitric oxide do not. Superoxide inhibits ECE by ejecting zinc from the enzyme, and the addition of exogenous zinc restores enzymatic activity. Superoxide may inhibit other zinc metalloproteinases by a similar mechanism and may thus play an important role in regulating the biology of blood vessels.

Original languageEnglish (US)
Pages (from-to)26423-26427
Number of pages5
JournalJournal of Biological Chemistry
Volume275
Issue number34
DOIs
StatePublished - Aug 25 2000

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Endothelins
Superoxides
Enzyme activity
Zinc
Reactive Oxygen Species
Enzymes
Cardiovascular system
Endothelial cells
Blood vessels
Metalloproteases
Endothelin-1
Cardiovascular System
Hydrogen Peroxide
Blood Vessels
Nitric Oxide
Endothelial Cells
Cell Proliferation
Molecules
Endothelin-Converting Enzymes
Water

ASJC Scopus subject areas

  • Biochemistry

Cite this

López-Ongil, S., Senchak, V., Saura, M., Zaragoza, C., Ames, M., Ballermann, B., ... Lowensteini, C. J. (2000). Superoxide regulation of endothelin-converting enzyme. Journal of Biological Chemistry, 275(34), 26423-26427. https://doi.org/10.1074/jbc.M000767200

Superoxide regulation of endothelin-converting enzyme. / López-Ongil, Susana; Senchak, Veronica; Saura, Marta; Zaragoza, Carlos; Ames, Michael; Ballermann, Barbara; Rodríguez-Puyol, Manuel; Rodríguez-Puyol, Diego; Lowensteini, Charles J.

In: Journal of Biological Chemistry, Vol. 275, No. 34, 25.08.2000, p. 26423-26427.

Research output: Contribution to journalArticle

López-Ongil, S, Senchak, V, Saura, M, Zaragoza, C, Ames, M, Ballermann, B, Rodríguez-Puyol, M, Rodríguez-Puyol, D & Lowensteini, CJ 2000, 'Superoxide regulation of endothelin-converting enzyme', Journal of Biological Chemistry, vol. 275, no. 34, pp. 26423-26427. https://doi.org/10.1074/jbc.M000767200
López-Ongil S, Senchak V, Saura M, Zaragoza C, Ames M, Ballermann B et al. Superoxide regulation of endothelin-converting enzyme. Journal of Biological Chemistry. 2000 Aug 25;275(34):26423-26427. https://doi.org/10.1074/jbc.M000767200
López-Ongil, Susana ; Senchak, Veronica ; Saura, Marta ; Zaragoza, Carlos ; Ames, Michael ; Ballermann, Barbara ; Rodríguez-Puyol, Manuel ; Rodríguez-Puyol, Diego ; Lowensteini, Charles J. / Superoxide regulation of endothelin-converting enzyme. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 34. pp. 26423-26427.
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