Superoxide Flashes in Single Mitochondria

Wang Wang, Huaqiang Fang, Linda Groom, Aiwu Cheng, Wanrui Zhang, Jie Liu, Xianhua Wang, Kaitao Li, Peidong Han, Ming Zheng, Jinhu Yin, Weidong Wang, Mark P. Mattson, Joseph P Y Kao, Edward Lakatta, Shey Shing Sheu, Kunfu Ouyang, Ju Chen, Robert T. Dirksen, Heping Cheng

Research output: Contribution to journalArticle

Abstract

In quiescent cells, mitochondria are the primary source of reactive oxygen species (ROS), which are generated by leakiness of the electron transport chain (ETC). High levels of ROS can trigger cell death, whereas lower levels drive diverse and important cellular functions. We show here by employing a newly developed mitochondrial matrix-targeted superoxide indicator, that individual mitochondria undergo spontaneous bursts of superoxide generation, termed "superoxide flashes." Superoxide flashes occur randomly in space and time, exhibit all-or-none properties, and provide a vital source of superoxide production across many different cell types. Individual flashes are triggered by transient openings of the mitochondrial permeability transition pore stimulating superoxide production by the ETC. Furthermore, we observe a flurry of superoxide flash activity during reoxygenation of cardiomyocytes after hypoxia, which is inhibited by the cardioprotective compound adenosine. We propose that superoxide flashes could serve as a valuable biomarker for a wide variety of oxidative stress-related diseases.

Original languageEnglish (US)
Pages (from-to)279-290
Number of pages12
JournalCell
Volume134
Issue number2
DOIs
StatePublished - Jul 25 2008
Externally publishedYes

Fingerprint

Mitochondria
Superoxides
Electron Transport
Reactive Oxygen Species
Oxidative stress
Biomarkers
Cell death
Cardiac Myocytes
Adenosine
Oxidative Stress
Cell Death

Keywords

  • CELLBIO
  • SIGNALING

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Wang, W., Fang, H., Groom, L., Cheng, A., Zhang, W., Liu, J., ... Cheng, H. (2008). Superoxide Flashes in Single Mitochondria. Cell, 134(2), 279-290. https://doi.org/10.1016/j.cell.2008.06.017

Superoxide Flashes in Single Mitochondria. / Wang, Wang; Fang, Huaqiang; Groom, Linda; Cheng, Aiwu; Zhang, Wanrui; Liu, Jie; Wang, Xianhua; Li, Kaitao; Han, Peidong; Zheng, Ming; Yin, Jinhu; Wang, Weidong; Mattson, Mark P.; Kao, Joseph P Y; Lakatta, Edward; Sheu, Shey Shing; Ouyang, Kunfu; Chen, Ju; Dirksen, Robert T.; Cheng, Heping.

In: Cell, Vol. 134, No. 2, 25.07.2008, p. 279-290.

Research output: Contribution to journalArticle

Wang, W, Fang, H, Groom, L, Cheng, A, Zhang, W, Liu, J, Wang, X, Li, K, Han, P, Zheng, M, Yin, J, Wang, W, Mattson, MP, Kao, JPY, Lakatta, E, Sheu, SS, Ouyang, K, Chen, J, Dirksen, RT & Cheng, H 2008, 'Superoxide Flashes in Single Mitochondria', Cell, vol. 134, no. 2, pp. 279-290. https://doi.org/10.1016/j.cell.2008.06.017
Wang W, Fang H, Groom L, Cheng A, Zhang W, Liu J et al. Superoxide Flashes in Single Mitochondria. Cell. 2008 Jul 25;134(2):279-290. https://doi.org/10.1016/j.cell.2008.06.017
Wang, Wang ; Fang, Huaqiang ; Groom, Linda ; Cheng, Aiwu ; Zhang, Wanrui ; Liu, Jie ; Wang, Xianhua ; Li, Kaitao ; Han, Peidong ; Zheng, Ming ; Yin, Jinhu ; Wang, Weidong ; Mattson, Mark P. ; Kao, Joseph P Y ; Lakatta, Edward ; Sheu, Shey Shing ; Ouyang, Kunfu ; Chen, Ju ; Dirksen, Robert T. ; Cheng, Heping. / Superoxide Flashes in Single Mitochondria. In: Cell. 2008 ; Vol. 134, No. 2. pp. 279-290.
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