TY - JOUR
T1 - Superior mesenteric artery occlusion models shock-induced gut ischemia-reperfusion
AU - Kozar, Rosemary A.
AU - Holcomb, John B.
AU - Hassoun, Heitham T.
AU - MacAitis, Joseph
AU - DeSoignie, Roland
AU - Moore, Frederick A.
N1 - Funding Information:
Supported by grants from the National Institutes of Health NIGMS Grant P50 GM 38529 and K08 GM62975 (RAK)
PY - 2004/1
Y1 - 2004/1
N2 - Background. Superior mesenteric artery occlusion (SMAO) is a simple and reproducible model of shock-induced gut ischemia/reperfusion, but some argue that it is not clinically relevant. The purpose of the current study was to compare SMAO to a standard model of controlled hemorrhage (CH) and uncontrolled hemorrhage (UH). Methods. Rats had femoral lines and a jejunal mucosal laser Doppler placed followed by SMAO (60 min of ischemia, no resuscitation), controlled hemorrhage (40 mm Hg for 60 min, 2:1 resuscitation shed blood and lactated Ringers), or uncontrolled hemorrhage (liver injury, 3:1 resuscitation with lactated Ringers). Base deficit, lactate, and jejunal mucosal flow (as a percentage of baseline) were recorded during ischemia and for 120 min after reperfusion. Jejunal tissue was harvested for morphological evaluation. Comparison among groups was by analysis of variance (ANOVA), and significance was set at P < 0.05. Results. Mucosal blood flow was similar among groups at the onset of reperfusion (CH, 16.9 ± 5.0% versus UH, 10.9 ± 3.1% versus SMAO, 13.9 ± 6.2%) and during the initial period of reperfusion. By 120 min, however, flow in CH (75.4 ± 2.5%) was significantly higher that in either UH (36.4 ± 13.1%) or SMAO (31.7 ± 8.4%). Histological injury was less with CH, while base deficit was significantly higher in CH at the onset of reperfusion (-24 ± 2 versus UH, -10 ± 3 and SMAO, -6 ± 3 mM/L) but comparable by the end (CH, -17 ± 4 versus UH, -16 ± 3 and SMAO, -17 ± 2 mM/L). Conclusion. SMAO is a clinically relevant model of shock-induced gut ischemia/reperfusion.
AB - Background. Superior mesenteric artery occlusion (SMAO) is a simple and reproducible model of shock-induced gut ischemia/reperfusion, but some argue that it is not clinically relevant. The purpose of the current study was to compare SMAO to a standard model of controlled hemorrhage (CH) and uncontrolled hemorrhage (UH). Methods. Rats had femoral lines and a jejunal mucosal laser Doppler placed followed by SMAO (60 min of ischemia, no resuscitation), controlled hemorrhage (40 mm Hg for 60 min, 2:1 resuscitation shed blood and lactated Ringers), or uncontrolled hemorrhage (liver injury, 3:1 resuscitation with lactated Ringers). Base deficit, lactate, and jejunal mucosal flow (as a percentage of baseline) were recorded during ischemia and for 120 min after reperfusion. Jejunal tissue was harvested for morphological evaluation. Comparison among groups was by analysis of variance (ANOVA), and significance was set at P < 0.05. Results. Mucosal blood flow was similar among groups at the onset of reperfusion (CH, 16.9 ± 5.0% versus UH, 10.9 ± 3.1% versus SMAO, 13.9 ± 6.2%) and during the initial period of reperfusion. By 120 min, however, flow in CH (75.4 ± 2.5%) was significantly higher that in either UH (36.4 ± 13.1%) or SMAO (31.7 ± 8.4%). Histological injury was less with CH, while base deficit was significantly higher in CH at the onset of reperfusion (-24 ± 2 versus UH, -10 ± 3 and SMAO, -6 ± 3 mM/L) but comparable by the end (CH, -17 ± 4 versus UH, -16 ± 3 and SMAO, -17 ± 2 mM/L). Conclusion. SMAO is a clinically relevant model of shock-induced gut ischemia/reperfusion.
KW - Controlled hemorrhage
KW - Ischemia/reperfusion
KW - Rodent model
KW - Shock
KW - Superior mesenteric artery occlusion
KW - Uncontrolled hemorrhage
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U2 - 10.1016/S0022-4804(03)00301-9
DO - 10.1016/S0022-4804(03)00301-9
M3 - Article
C2 - 14732361
AN - SCOPUS:0347721109
SN - 0022-4804
VL - 116
SP - 145
EP - 150
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 1
ER -