Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging

Amnon Bar-Shir, Yajie Liang, Kannie W Y Chan, Assaf A. Gilad, Jeff W Bulte

Research output: Contribution to journalArticle

Abstract

Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.

Original languageEnglish (US)
Pages (from-to)4869-4871
Number of pages3
JournalChemical Communications
Volume51
Issue number23
DOIs
StatePublished - Mar 21 2015

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Green Fluorescent Proteins
Magnetic resonance imaging
Genes
Proteins
Imaging techniques
Fluorescence
Cells

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Metals and Alloys

Cite this

Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging. / Bar-Shir, Amnon; Liang, Yajie; Chan, Kannie W Y; Gilad, Assaf A.; Bulte, Jeff W.

In: Chemical Communications, Vol. 51, No. 23, 21.03.2015, p. 4869-4871.

Research output: Contribution to journalArticle

Bar-Shir, Amnon ; Liang, Yajie ; Chan, Kannie W Y ; Gilad, Assaf A. ; Bulte, Jeff W. / Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging. In: Chemical Communications. 2015 ; Vol. 51, No. 23. pp. 4869-4871.
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