Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging

Amnon Bar-Shir, Yajie Liang, Kannie W.Y. Chan, Assaf A. Gilad, Jeff W.M. Bulte

Research output: Contribution to journalArticle

Abstract

Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.

Original languageEnglish (US)
Pages (from-to)4869-4871
Number of pages3
JournalChemical Communications
Volume51
Issue number23
DOIs
StatePublished - Mar 21 2015

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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