Superantigen enhanced protection against a weak tumor-specific melanoma antigen: Implications for prophylactic vaccination against cancer

Scott L. Kominsky, Barbara A. Torres, Amy C. Hobeika, Faith A. Lake, Howard M. Johnson

Research output: Contribution to journalArticlepeer-review

Abstract

B16F10 melanoma is a tumor derived from C57BL/6 mice that has been found to be poorly immunogenic and highly aggressive. Here we have shown that vaccination of mice with irradiated B16F10 cells followed by treatment with a combination of staphylococcal enterotoxins A and B (SEA/SEB) leads to significant and specific protection against subsequent challenge with viable B16F10 cells (at least 25-fold greater than a lethal dose). Also, 75% of mice surviving over 150 days remained tumor-free after rechallenge with viable B16F10 cells, evidence of the development of strong immunologic memory. Additional studies showed increases in CD4+ and CD8+ T-cell populations, cytotoxic T-lymphocyte activity and interferon-γ production, all of which may contribute to enhanced survival. Furthermore, failure to produce protection in either CD4-/- or CD8-/- T-cell knockout mice is evidence that CD4+ and CD8+ T cells play an essential role in induction of immunity. These results show that superantigen administration subsequent to vaccination with inactivated tumor cells results in protective antitumor immunity. Thus, prophylactic vaccination against cancer is a feasible method for arming the immune system prior to the incidence of cancer.

Original languageEnglish (US)
Pages (from-to)834-841
Number of pages8
JournalInternational Journal of Cancer
Volume94
Issue number6
DOIs
StatePublished - Dec 15 2001
Externally publishedYes

Keywords

  • Antitumor immunity
  • Cell cycle
  • Melanoma
  • Superantigens
  • Vaccination

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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