Super-resolution two-photon microscopy via scanning patterned illumination

Ben E. Urban; Ji Yi; Siyu Chen; Biqin Dong; Yongling Zhu; Steven H. Devries; Vadim Backman; Hao F. Zhang

doi:10.1103/PhysRevE.91.042703

Super-resolution two-photon microscopy via scanning patterned illumination

Ben E. Urban, Ji Yi, Siyu Chen, Biqin Dong, Yongling Zhu, Steven H. Devries, Vadim Backman, Hao F. Zhang

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We developed two-photon scanning patterned illumination microscopy (2P-SPIM) for super-resolution two-photon imaging. Our approach used a traditional two-photon microscopy setup with temporally modulated excitation to create patterned illumination fields. Combing nine different illuminations and structured illumination reconstruction, super-resolution imaging was achieved in two-photon microscopy. Using 2P-SPIM we achieved a lateral resolution of 141 nm, which represents an improvement by a factor of 1.9 over the corresponding diffraction limit. We further demonstrated super-resolution cellular imaging by 2P-SPIM to image actin cytoskeleton in mammalian cells and three-dimensional imaging in highly scattering retinal tissue.

Original language	English (US)
Article number	042703
Journal	Physical Review E - Statistical, Nonlinear, and Soft Matter Physics
Volume	91
Issue number	4
DOIs	https://doi.org/10.1103/PhysRevE.91.042703
State	Published - Apr 7 2015
Externally published	Yes

ASJC Scopus subject areas

Statistical and Nonlinear Physics
Statistics and Probability
Condensed Matter Physics

Access to Document

10.1103/PhysRevE.91.042703

Cite this

@article{ac003bd1d8134988a498df879639f707,

title = "Super-resolution two-photon microscopy via scanning patterned illumination",

abstract = "We developed two-photon scanning patterned illumination microscopy (2P-SPIM) for super-resolution two-photon imaging. Our approach used a traditional two-photon microscopy setup with temporally modulated excitation to create patterned illumination fields. Combing nine different illuminations and structured illumination reconstruction, super-resolution imaging was achieved in two-photon microscopy. Using 2P-SPIM we achieved a lateral resolution of 141 nm, which represents an improvement by a factor of 1.9 over the corresponding diffraction limit. We further demonstrated super-resolution cellular imaging by 2P-SPIM to image actin cytoskeleton in mammalian cells and three-dimensional imaging in highly scattering retinal tissue.",

author = "Urban, {Ben E.} and Ji Yi and Siyu Chen and Biqin Dong and Yongling Zhu and Devries, {Steven H.} and Vadim Backman and Zhang, {Hao F.}",

note = "Publisher Copyright: {\textcopyright} 2015 American Physical Society.",

year = "2015",

month = apr,

day = "7",

doi = "10.1103/PhysRevE.91.042703",

language = "English (US)",

volume = "91",

journal = "Physical Review E - Statistical, Nonlinear, and Soft Matter Physics",

issn = "1539-3755",

publisher = "American Physical Society",

number = "4",

}

TY - JOUR

T1 - Super-resolution two-photon microscopy via scanning patterned illumination

AU - Urban, Ben E.

AU - Yi, Ji

AU - Chen, Siyu

AU - Dong, Biqin

AU - Zhu, Yongling

AU - Devries, Steven H.

AU - Backman, Vadim

AU - Zhang, Hao F.

PY - 2015/4/7

Y1 - 2015/4/7

N2 - We developed two-photon scanning patterned illumination microscopy (2P-SPIM) for super-resolution two-photon imaging. Our approach used a traditional two-photon microscopy setup with temporally modulated excitation to create patterned illumination fields. Combing nine different illuminations and structured illumination reconstruction, super-resolution imaging was achieved in two-photon microscopy. Using 2P-SPIM we achieved a lateral resolution of 141 nm, which represents an improvement by a factor of 1.9 over the corresponding diffraction limit. We further demonstrated super-resolution cellular imaging by 2P-SPIM to image actin cytoskeleton in mammalian cells and three-dimensional imaging in highly scattering retinal tissue.

AB - We developed two-photon scanning patterned illumination microscopy (2P-SPIM) for super-resolution two-photon imaging. Our approach used a traditional two-photon microscopy setup with temporally modulated excitation to create patterned illumination fields. Combing nine different illuminations and structured illumination reconstruction, super-resolution imaging was achieved in two-photon microscopy. Using 2P-SPIM we achieved a lateral resolution of 141 nm, which represents an improvement by a factor of 1.9 over the corresponding diffraction limit. We further demonstrated super-resolution cellular imaging by 2P-SPIM to image actin cytoskeleton in mammalian cells and three-dimensional imaging in highly scattering retinal tissue.

UR - http://www.scopus.com/inward/record.url?scp=84929095475&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929095475&partnerID=8YFLogxK

U2 - 10.1103/PhysRevE.91.042703

DO - 10.1103/PhysRevE.91.042703

M3 - Article

C2 - 25974523

AN - SCOPUS:84929095475

SN - 1539-3755

VL - 91

JO - Physical Review E - Statistical, Nonlinear, and Soft Matter Physics

JF - Physical Review E - Statistical, Nonlinear, and Soft Matter Physics

IS - 4

M1 - 042703

ER -

Super-resolution two-photon microscopy via scanning patterned illumination

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this