[3H]ketanserin binding increases in monkey cortex following basal forebrain lesions with ibotenic acid

Gary L. Wenk; Kathrin L. Engisch; Lisa D. McCall; Susan J. Mitchell; Thomas G. Aigner; Robert L. Struble; Donald L. Price; David S. Olton

doi:10.1016/0197-0186(86)90150-6

[³H]ketanserin binding increases in monkey cortex following basal forebrain lesions with ibotenic acid

Gary L. Wenk, Kathrin L. Engisch, Lisa D. McCall, Susan J. Mitchell, Thomas G. Aigner, Robert L. Struble, Donald L. Price, David S. Olton

School of Medicine

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The present study investigated the effects of damage to the basal forebrain cholinergic system upon [³H]ketanserin binding in the neocortex and hippocampus of monkeys. [³H]Ketanserin specifically binds to serotonin type-2 receptor sites. Lesions were placed in the medial septal area, nucleus basalis, or both regions. Ten months later, [³H]ketanserin binding was increased in the neocortex, but not in the hippocampus, while levels of choline acetyltransferase (acetyl-CoA: choline O-acetyltransferase, EC 2.3.1.6) activity decreased in the neocortex and hippocampus. Changes in the levels of choline acetyltransferase and [³H]ketanserin binding were correlated significantly in the neocortex (r = -0.64, P < 0.025), but not in the hippocampus. The data suggest that degeneration of the basal forebrain cholinergic system may alter serotonergic function in the neocortex.

Original language	English (US)
Pages (from-to)	557-562
Number of pages	6
Journal	Neurochemistry International
Volume	9
Issue number	4
DOIs	https://doi.org/10.1016/0197-0186(86)90150-6
State	Published - 1986

ASJC Scopus subject areas

Cellular and Molecular Neuroscience
Cell Biology

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@article{6b8517f1aece4ca58ed908e8ded70493,

title = "[3H]ketanserin binding increases in monkey cortex following basal forebrain lesions with ibotenic acid",

abstract = "The present study investigated the effects of damage to the basal forebrain cholinergic system upon [3H]ketanserin binding in the neocortex and hippocampus of monkeys. [3H]Ketanserin specifically binds to serotonin type-2 receptor sites. Lesions were placed in the medial septal area, nucleus basalis, or both regions. Ten months later, [3H]ketanserin binding was increased in the neocortex, but not in the hippocampus, while levels of choline acetyltransferase (acetyl-CoA: choline O-acetyltransferase, EC 2.3.1.6) activity decreased in the neocortex and hippocampus. Changes in the levels of choline acetyltransferase and [3H]ketanserin binding were correlated significantly in the neocortex (r = -0.64, P < 0.025), but not in the hippocampus. The data suggest that degeneration of the basal forebrain cholinergic system may alter serotonergic function in the neocortex.",

author = "Wenk, {Gary L.} and Engisch, {Kathrin L.} and McCall, {Lisa D.} and Mitchell, {Susan J.} and Aigner, {Thomas G.} and Struble, {Robert L.} and Price, {Donald L.} and Olton, {David S.}",

note = "Funding Information: Acknowledgements--This research was supported in part by a grant from the U.S. Army Medical Research and Development Command, Contract No. DAMD17-82-C-2225 and by funds from the U.S. Public Health Service (NIH AG 05146 and NS 20471). The authors thank M. Mishkin for supplying the monkey tissue, P. Hartig for technical comments on the binding assay, and A. Fagan, D. Hughey, M. Shapiro, C. Wible and C. Raphael for critically reading the manuscript.",

year = "1986",

doi = "10.1016/0197-0186(86)90150-6",

language = "English (US)",

volume = "9",

pages = "557--562",

journal = "Neurochemistry International",

issn = "0197-0186",

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AU - Wenk, Gary L.

AU - Engisch, Kathrin L.

AU - McCall, Lisa D.

AU - Mitchell, Susan J.

AU - Aigner, Thomas G.

AU - Struble, Robert L.

AU - Price, Donald L.

AU - Olton, David S.

N1 - Funding Information: Acknowledgements--This research was supported in part by a grant from the U.S. Army Medical Research and Development Command, Contract No. DAMD17-82-C-2225 and by funds from the U.S. Public Health Service (NIH AG 05146 and NS 20471). The authors thank M. Mishkin for supplying the monkey tissue, P. Hartig for technical comments on the binding assay, and A. Fagan, D. Hughey, M. Shapiro, C. Wible and C. Raphael for critically reading the manuscript.

PY - 1986

Y1 - 1986

N2 - The present study investigated the effects of damage to the basal forebrain cholinergic system upon [3H]ketanserin binding in the neocortex and hippocampus of monkeys. [3H]Ketanserin specifically binds to serotonin type-2 receptor sites. Lesions were placed in the medial septal area, nucleus basalis, or both regions. Ten months later, [3H]ketanserin binding was increased in the neocortex, but not in the hippocampus, while levels of choline acetyltransferase (acetyl-CoA: choline O-acetyltransferase, EC 2.3.1.6) activity decreased in the neocortex and hippocampus. Changes in the levels of choline acetyltransferase and [3H]ketanserin binding were correlated significantly in the neocortex (r = -0.64, P < 0.025), but not in the hippocampus. The data suggest that degeneration of the basal forebrain cholinergic system may alter serotonergic function in the neocortex.

AB - The present study investigated the effects of damage to the basal forebrain cholinergic system upon [3H]ketanserin binding in the neocortex and hippocampus of monkeys. [3H]Ketanserin specifically binds to serotonin type-2 receptor sites. Lesions were placed in the medial septal area, nucleus basalis, or both regions. Ten months later, [3H]ketanserin binding was increased in the neocortex, but not in the hippocampus, while levels of choline acetyltransferase (acetyl-CoA: choline O-acetyltransferase, EC 2.3.1.6) activity decreased in the neocortex and hippocampus. Changes in the levels of choline acetyltransferase and [3H]ketanserin binding were correlated significantly in the neocortex (r = -0.64, P < 0.025), but not in the hippocampus. The data suggest that degeneration of the basal forebrain cholinergic system may alter serotonergic function in the neocortex.

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[³H]ketanserin binding increases in monkey cortex following basal forebrain lesions with ibotenic acid

Abstract

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