31P NMR of mammalian cells encapsulated in alginate gels utilizing a new phosphate‐free perfusion medium

K. Shankar Narayan, E. A. Moress, J. C. Chatham, P. B. Barker

Research output: Contribution to journalArticlepeer-review

Abstract

A simple technique permitting long term 31P magnetic resonance spectroscopy of cultured mammalian cells is described. Cells are encapsulated in calcium alginate gels and perfused with a new phosphate‐free medium designed for maintenance of high cell viability. Intracellular pH and β‐NTP/Pi ratios, studied in three cell types, remained stable over a 16–20 h NMR monitoring period. The techniques described may be useful for monitoring cell metabolism with a variety of cell types.

Original languageEnglish (US)
Pages (from-to)23-26
Number of pages4
JournalNMR in biomedicine
Volume3
Issue number1
DOIs
StatePublished - Feb 1990

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

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