1H NMR spectroscopy in the diagnosis of Pseudomonas aeruginosa-induced urinary tract infection

Ashish Gupta, Mayank Dwivedi, G. A. Nagana Gowda, Archana Ayyagari, A. A. Mahdi, M. Bhandari, C. L. Khetrapal

Research output: Contribution to journalArticlepeer-review

Abstract

The utility of 1H NMR spectroscopy is suggested and demonstrated for the diagnosis of Pseudomonas aeruginosa in urinary tract infection (UTI). The specific property of P. aeruginosa of metabolizing nicotinic acid to 6-hydroxynicotinic acid (6-OHNA) is exploited. The quantity of 6-OHNA produced correlates well with the viable bacterial count. Other common bacteria causing UTI such as Escherichia coli, Klebsiella pneumonia, Enterobacter aerogenes, Acinetobacter baumanii, Proteus mirabilis, Citrobacter frundii, Enterococcus faecalis, Streptococcus gp B and Staphylococcus aureus do not metabolize nicotinic acid under similar conditions. The method provides a single-step documentation of P. aeruginosa qualitatively as well as quantitatively. The NMR method is demonstrated on urine samples from 30 patients with UTI caused by P. aeruginosa.

Original languageEnglish (US)
Pages (from-to)293-299
Number of pages7
JournalNMR in biomedicine
Volume18
Issue number5
DOIs
StatePublished - Aug 1 2005

Keywords

  • Diagnosis
  • Infection
  • Proton magnetic resonance
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

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