Abstract
A two-step heteronuclear enhancement approach was combined with chemical exchange saturation transfer (CEST) to magnify 15N MRI signal of molecules through indirect detection via water protons. Previous CEST studies have been limited to radiofrequency (rf) saturation transfer or excitation transfer employing protons. Here, the signal of 15N is detected indirectly through the water signal by first inverting selectively protons that are scalar-coupled to 15N in the urea molecule, followed by chemical exchange of the amide proton to bulk water. In addition to providing a small sensitivity enhancement, this approach can be used to monitor the exchange rates and thus the pH sensitivity of the participating 15N-bound protons.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 11136-11139 |
| Number of pages | 4 |
| Journal | Journal of the American Chemical Society |
| Volume | 138 |
| Issue number | 35 |
| DOIs | |
| State | Published - Sep 7 2016 |
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ASJC Scopus subject areas
- Catalysis
- Biochemistry
- Chemistry(all)
- Colloid and Surface Chemistry
Cite this
15N Heteronuclear Chemical Exchange Saturation Transfer MRI. / Zeng, Haifeng; Xu, Jiadi; Yadav, Nirbhay N.; Mcmahon, Michael T; Harden, Bradley; Frueh, Dominique P; Van Zijl, Peter C.
In: Journal of the American Chemical Society, Vol. 138, No. 35, 07.09.2016, p. 11136-11139.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 15N Heteronuclear Chemical Exchange Saturation Transfer MRI
AU - Zeng, Haifeng
AU - Xu, Jiadi
AU - Yadav, Nirbhay N.
AU - Mcmahon, Michael T
AU - Harden, Bradley
AU - Frueh, Dominique P
AU - Van Zijl, Peter C
PY - 2016/9/7
Y1 - 2016/9/7
N2 - A two-step heteronuclear enhancement approach was combined with chemical exchange saturation transfer (CEST) to magnify 15N MRI signal of molecules through indirect detection via water protons. Previous CEST studies have been limited to radiofrequency (rf) saturation transfer or excitation transfer employing protons. Here, the signal of 15N is detected indirectly through the water signal by first inverting selectively protons that are scalar-coupled to 15N in the urea molecule, followed by chemical exchange of the amide proton to bulk water. In addition to providing a small sensitivity enhancement, this approach can be used to monitor the exchange rates and thus the pH sensitivity of the participating 15N-bound protons.
AB - A two-step heteronuclear enhancement approach was combined with chemical exchange saturation transfer (CEST) to magnify 15N MRI signal of molecules through indirect detection via water protons. Previous CEST studies have been limited to radiofrequency (rf) saturation transfer or excitation transfer employing protons. Here, the signal of 15N is detected indirectly through the water signal by first inverting selectively protons that are scalar-coupled to 15N in the urea molecule, followed by chemical exchange of the amide proton to bulk water. In addition to providing a small sensitivity enhancement, this approach can be used to monitor the exchange rates and thus the pH sensitivity of the participating 15N-bound protons.
UR - http://www.scopus.com/inward/record.url?scp=84986229696&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84986229696&partnerID=8YFLogxK
U2 - 10.1021/jacs.6b06421
DO - 10.1021/jacs.6b06421
M3 - Article
C2 - 27548755
AN - SCOPUS:84986229696
VL - 138
SP - 11136
EP - 11139
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 35
ER -