15N Heteronuclear Chemical Exchange Saturation Transfer MRI

Haifeng Zeng, Jiadi Xu, Nirbhay N. Yadav, Michael T. McMahon, Bradley Harden, Dominique Frueh, Peter C M Van Zijl

Research output: Contribution to journalArticle

Abstract

A two-step heteronuclear enhancement approach was combined with chemical exchange saturation transfer (CEST) to magnify 15N MRI signal of molecules through indirect detection via water protons. Previous CEST studies have been limited to radiofrequency (rf) saturation transfer or excitation transfer employing protons. Here, the signal of 15N is detected indirectly through the water signal by first inverting selectively protons that are scalar-coupled to 15N in the urea molecule, followed by chemical exchange of the amide proton to bulk water. In addition to providing a small sensitivity enhancement, this approach can be used to monitor the exchange rates and thus the pH sensitivity of the participating 15N-bound protons.

LanguageEnglish (US)
Pages11136-11139
Number of pages4
JournalJournal of the American Chemical Society
Volume138
Issue number35
DOIs
StatePublished - Sep 7 2016

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Magnetic resonance imaging
Protons
Water
Molecules
Proton transfer
Amides
Urea
Ion exchange

ASJC Scopus subject areas

  • Catalysis
  • Biochemistry
  • Chemistry(all)
  • Colloid and Surface Chemistry

Cite this

15N Heteronuclear Chemical Exchange Saturation Transfer MRI. / Zeng, Haifeng; Xu, Jiadi; Yadav, Nirbhay N.; McMahon, Michael T.; Harden, Bradley; Frueh, Dominique; Van Zijl, Peter C M.

In: Journal of the American Chemical Society, Vol. 138, No. 35, 07.09.2016, p. 11136-11139.

Research output: Contribution to journalArticle

Zeng H, Xu J, Yadav NN, McMahon MT, Harden B, Frueh D et al. 15N Heteronuclear Chemical Exchange Saturation Transfer MRI. Journal of the American Chemical Society. 2016 Sep 7;138(35):11136-11139. Available from, DOI: 10.1021/jacs.6b06421
Zeng, Haifeng ; Xu, Jiadi ; Yadav, Nirbhay N. ; McMahon, Michael T. ; Harden, Bradley ; Frueh, Dominique ; Van Zijl, Peter C M. / 15N Heteronuclear Chemical Exchange Saturation Transfer MRI. In: Journal of the American Chemical Society. 2016 ; Vol. 138, No. 35. pp. 11136-11139
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