Abstract
We have monitored the binding of [125I]thienylphencyclidine ([125I]TCP), a novel high affinity radioiodinated ligand that specifically recognizes the NMDA (N-methyl-D-aspartate) receptor in rat brain membranes. [125I]TCP binds with an affinity of about 30 nM, and recognizes a similar number of binding sites to previously employed ligands for this receptor. [125I]TCP binding is characterized by slow association and dissociation rates, and the latter can be modified by the addition of Mg2+ or Zn2+, as previously described for [3H]dizocilpine ([3MK801). Other phencylidine-like ligands displaced [125I]TCP binding with the order of potency dizocilpine > thienylphencyclidine > ITCP > phencyclidine > ketamine. The binding of [125I]TCP was also increased by NMDA and glycine-site agonists and inhibited by antagonists of these sites. Surprisingly, however, the polyamines spermidine and spermine did not increase [125I]TCP, even though the polyamine antagonist arcaine was an effective inhibitor of binding. These results show that [125I]TCP is a useful ligand for the NMDA receptor complex that binds to the receptor in a manner that is qualitatively distinct from previously described ligands.
Original language | English (US) |
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Pages (from-to) | 53-58 |
Number of pages | 6 |
Journal | European Journal of Pharmacology: Molecular Pharmacology |
Volume | 226 |
Issue number | 1 |
DOIs | |
State | Published - May 12 1992 |
Keywords
- NMDA receptor
- Polyamines
- [I]Thienylphencyclidinc
ASJC Scopus subject areas
- Pharmacology