SUN-1 and ZYG-12, mediators of centrosome-nucleus attachment, are a functional SUN/KASH pair in Caenorhabditis elegans

Il Minn, Melissa M. Rolls, Wendy Hanna-Rose, Christian J. Malone

Research output: Contribution to journalArticle

Abstract

Klarsicht/ANC-1/Syne/homology (KASH)/Sad-1/UNC-84 (SUN) protein pairs can act as connectors between cytoplasmic organelles and the nucleoskeleton. Caenorhabditis elegans ZYG-12 and SUN-1 are essential for centrosome-nucleus attachment. Although SUN-1 has a canonical SUN domain, ZYG-12 has a divergent KASH domain. Here, we establish that the ZYG-12 mini KASH domain is functional and, in combination with a portion of coiled-coil domain, is sufficient for nuclear envelope localization. ZYG-12 and SUN-1 are hypothesized to be outer and inner nuclear membrane proteins, respectively, and to interact, but neither their topologies nor their physical interaction has been directly investigated. We show that ZYG-12 is a type II outer nuclear membrane (ONM) protein and that SUN-1 is a type II inner nuclear membrane protein. The proteins interact in the luminal space of the nuclear envelope via the ZYG-12 mini KASH domain and a region of SUN-1 that does not include the SUN domain. SUN-1 is hypothesized to restrict ZYG-12 to the ONM, preventing diffusion through the endoplasmic reticulum. We establish that ZYG-12 is indeed immobile at the ONM by using fluorescence recovery after photobleaching and show that SUN-1 is sufficient to localize ZYG-12 in cells. This work supports current models of KASH/SUN pairs and highlights the diversity in sequence elements defining KASH domains.

Original languageEnglish (US)
Pages (from-to)4586-4595
Number of pages10
JournalMolecular Biology of the Cell
Volume20
Issue number21
DOIs
StatePublished - 2009
Externally publishedYes

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Centrosome
Nuclear Envelope
Caenorhabditis elegans
Nuclear Proteins
Membrane Proteins
Fluorescence Recovery After Photobleaching
Nuclear Matrix
ANC 1
Endoplasmic Reticulum
Organelles
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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SUN-1 and ZYG-12, mediators of centrosome-nucleus attachment, are a functional SUN/KASH pair in Caenorhabditis elegans. / Minn, Il; Rolls, Melissa M.; Hanna-Rose, Wendy; Malone, Christian J.

In: Molecular Biology of the Cell, Vol. 20, No. 21, 2009, p. 4586-4595.

Research output: Contribution to journalArticle

Minn, Il ; Rolls, Melissa M. ; Hanna-Rose, Wendy ; Malone, Christian J. / SUN-1 and ZYG-12, mediators of centrosome-nucleus attachment, are a functional SUN/KASH pair in Caenorhabditis elegans. In: Molecular Biology of the Cell. 2009 ; Vol. 20, No. 21. pp. 4586-4595.
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