SUMO-1 protease-1 regulates gene transcription through PML

Jennifer L. Best; Soula Ganiatsas; Sadhana Agarwal; Austin Changou; Paolo Salomoni; Orian Shirihai; Pamela B. Meluh; Pier Paolo Pandolfi; Leonard I. Zon

doi:10.1016/S1097-2765(02)00699-8

SUMO-1 protease-1 regulates gene transcription through PML

Jennifer L. Best, Soula Ganiatsas, Sadhana Agarwal, Austin Changou, Paolo Salomoni, Orian Shirihai, Pamela B. Meluh, Pier Paolo Pandolfi, Leonard I. Zon

Research output: Contribution to journal › Article › peer-review

147 Scopus citations

Abstract

During a screen to identify c-Jun activators, we isolated a cysteine protease, SuPr-1, that induced c-Jun-dependent transcription independently of c-Jun phosphorylation. SuPr-1 is a member of a new family of proteases that hydrolyze the ubiquitin-like modifier, SUMO-1. SuPr-1 hydrolyzed SUMO-1-modified forms of the promyelocytic leukemia gene product, PML, and altered the subcellular distribution of PML in nuclear PODs (PML oncogenic domains). SuPr-1 also altered the distribution of other nuclear POD-associated proteins, such as CBP and Daxx, that act as transcriptional regulators. SuPr-1 action on transcription was enhanced by PML, and SuPr-1 failed to activate transcription in PML-deficient fibroblasts. Our studies establish an important role for SUMO proteases in transcription.

Original language	English (US)
Pages (from-to)	843-855
Number of pages	13
Journal	Molecular cell
Volume	10
Issue number	4
DOIs	https://doi.org/10.1016/S1097-2765(02)00699-8
State	Published - Oct 1 2002
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/S1097-2765(02)00699-8

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title = "SUMO-1 protease-1 regulates gene transcription through PML",

abstract = "During a screen to identify c-Jun activators, we isolated a cysteine protease, SuPr-1, that induced c-Jun-dependent transcription independently of c-Jun phosphorylation. SuPr-1 is a member of a new family of proteases that hydrolyze the ubiquitin-like modifier, SUMO-1. SuPr-1 hydrolyzed SUMO-1-modified forms of the promyelocytic leukemia gene product, PML, and altered the subcellular distribution of PML in nuclear PODs (PML oncogenic domains). SuPr-1 also altered the distribution of other nuclear POD-associated proteins, such as CBP and Daxx, that act as transcriptional regulators. SuPr-1 action on transcription was enhanced by PML, and SuPr-1 failed to activate transcription in PML-deficient fibroblasts. Our studies establish an important role for SUMO proteases in transcription.",

author = "Best, {Jennifer L.} and Soula Ganiatsas and Sadhana Agarwal and Austin Changou and Paolo Salomoni and Orian Shirihai and Meluh, {Pamela B.} and Pandolfi, {Pier Paolo} and Zon, {Leonard I.}",

note = "Funding Information: We thank M. Montminy, B. Mayer, and A. Davidson for critical review of this manuscript; R. Davis, D. Pellman, Y. Zhou, T. Huber, A. Donovan, and K. Du for helpful discussions and experimental advice; D. Giarla for administrative assistance; S. Zhong and Ailan Guo for assistance with the PML −/− MEF transfections; and R. Hay, K. McBride, G. Blobel, and X. He for plasmid constructs. L.I.Z. is an Associate Investigator of the Howard Hughes Medical Institute. This work was supported by grants from the NIH.",

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doi = "10.1016/S1097-2765(02)00699-8",

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T1 - SUMO-1 protease-1 regulates gene transcription through PML

AU - Best, Jennifer L.

AU - Ganiatsas, Soula

AU - Agarwal, Sadhana

AU - Changou, Austin

AU - Salomoni, Paolo

AU - Shirihai, Orian

AU - Meluh, Pamela B.

AU - Pandolfi, Pier Paolo

AU - Zon, Leonard I.

N1 - Funding Information: We thank M. Montminy, B. Mayer, and A. Davidson for critical review of this manuscript; R. Davis, D. Pellman, Y. Zhou, T. Huber, A. Donovan, and K. Du for helpful discussions and experimental advice; D. Giarla for administrative assistance; S. Zhong and Ailan Guo for assistance with the PML −/− MEF transfections; and R. Hay, K. McBride, G. Blobel, and X. He for plasmid constructs. L.I.Z. is an Associate Investigator of the Howard Hughes Medical Institute. This work was supported by grants from the NIH.

PY - 2002/10/1

Y1 - 2002/10/1

N2 - During a screen to identify c-Jun activators, we isolated a cysteine protease, SuPr-1, that induced c-Jun-dependent transcription independently of c-Jun phosphorylation. SuPr-1 is a member of a new family of proteases that hydrolyze the ubiquitin-like modifier, SUMO-1. SuPr-1 hydrolyzed SUMO-1-modified forms of the promyelocytic leukemia gene product, PML, and altered the subcellular distribution of PML in nuclear PODs (PML oncogenic domains). SuPr-1 also altered the distribution of other nuclear POD-associated proteins, such as CBP and Daxx, that act as transcriptional regulators. SuPr-1 action on transcription was enhanced by PML, and SuPr-1 failed to activate transcription in PML-deficient fibroblasts. Our studies establish an important role for SUMO proteases in transcription.

AB - During a screen to identify c-Jun activators, we isolated a cysteine protease, SuPr-1, that induced c-Jun-dependent transcription independently of c-Jun phosphorylation. SuPr-1 is a member of a new family of proteases that hydrolyze the ubiquitin-like modifier, SUMO-1. SuPr-1 hydrolyzed SUMO-1-modified forms of the promyelocytic leukemia gene product, PML, and altered the subcellular distribution of PML in nuclear PODs (PML oncogenic domains). SuPr-1 also altered the distribution of other nuclear POD-associated proteins, such as CBP and Daxx, that act as transcriptional regulators. SuPr-1 action on transcription was enhanced by PML, and SuPr-1 failed to activate transcription in PML-deficient fibroblasts. Our studies establish an important role for SUMO proteases in transcription.

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JO - Molecular cell

JF - Molecular cell

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ER -

SUMO-1 protease-1 regulates gene transcription through PML

Abstract

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