SUMO-1 protease-1 regulates gene transcription through PML

Jennifer L. Best, Soula Ganiatsas, Sadhana Agarwal, Austin Changou, Paolo Salomoni, Orian Shirihai, Pamela B. Meluh, Pier Paolo Pandolfi, Leonard I. Zon

Research output: Contribution to journalArticlepeer-review

Abstract

During a screen to identify c-Jun activators, we isolated a cysteine protease, SuPr-1, that induced c-Jun-dependent transcription independently of c-Jun phosphorylation. SuPr-1 is a member of a new family of proteases that hydrolyze the ubiquitin-like modifier, SUMO-1. SuPr-1 hydrolyzed SUMO-1-modified forms of the promyelocytic leukemia gene product, PML, and altered the subcellular distribution of PML in nuclear PODs (PML oncogenic domains). SuPr-1 also altered the distribution of other nuclear POD-associated proteins, such as CBP and Daxx, that act as transcriptional regulators. SuPr-1 action on transcription was enhanced by PML, and SuPr-1 failed to activate transcription in PML-deficient fibroblasts. Our studies establish an important role for SUMO proteases in transcription.

Original languageEnglish (US)
Pages (from-to)843-855
Number of pages13
JournalMolecular cell
Volume10
Issue number4
DOIs
StatePublished - Oct 1 2002

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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