Sulfatide reduces leucocyte accumulation and reverts vascular failure in splanchnic artery occlusion shock

Francesco Squadrito, Domenica Altavilla, Giovanni Squadrito, Giuseppe M. Campo, Mariarita Arlotta, Cristina Quartarone, Letteria Minutoli, Marcella Ferlito, Antonino Saitta, Achille P. Caputi

Research output: Contribution to journalArticle

Abstract

Selectin-mediated leucocyte accumulation is implicated in the pathogenesis of splanchnic artery occlusion. Sulfatide is recognized by P-selectin and blocks this adhesion molecule. We investigated the effects of sulfatide in rats subjected to splanchnic artery occlusion shock. Anaesthetized rats, subjected to total occlusion of the superior mesenteric artery and the coeliac trunk for 45 min developed severe shock resulting in death within 85-90 min after the release of occlusion. Sham operated animals were used as controls. Splanchnic artery occlusion shocked rats had marked hypotension, enhanced levels of tumor necrosis factor-α (TNF-α) in serum and macrophages, leucopenia and increased ileal leucocyte accumulation, studied by the means of myeloperoxidase activity. Furthermore, aortae from shocked rats showed marked hyporeactivity to phenylephrine (1 nM-10 μM), reduced responsiveness to acetylcholine (10 nM-10 μM) and an increased staining for P-selectin in the vasculature. In vivo administration of sulfatide (10 mg/kg, i.v., 5 min after occlusion of the splanchnic arteries increased survival rate (90%, 4 h after splanchnic artery occlusion shock , enhanced mean arterial blood pressure,reduced serum TNF-a 3711 U r ml vs. 39818 U r ml , ameliorated leucopenia and reduced ileal myeloproxidase activity (1.2 ± 0.4 U/g tissue vs. 8.2 ± 0.8 U/g tissue). Aortae from splanchnic artery occlusion shocked rats treated with sulfatide exhibited a greater contractile response to phenylephrine and improved responsiveness to acetylcholine. Moreover sulfatide-treated rats showed a reduced staining for P-selectin in the aorta and in the superior mesenteric artery. Finally, passive immunization with specific monoclonal antibodies raised against P-selectin significantly protected from the lethality induced by splanchnic artery occlusion shock. Our results suggest that sulfatide protects against splanchnic artery occlusion shock. (C) 1998 Elsevier Science B.V. All rights reserved.

Original languageEnglish (US)
Pages (from-to)101-108
Number of pages8
JournalEuropean Journal of Pharmacology
Volume361
Issue number1
DOIs
StatePublished - Nov 13 1998
Externally publishedYes

Fingerprint

Sulfoglycosphingolipids
Viscera
Blood Vessels
Shock
Leukocytes
Arteries
P-Selectin
Aorta
Superior Mesenteric Artery
Leukopenia
Phenylephrine
Acetylcholine
Arterial Pressure
Tumor Necrosis Factor-alpha
Staining and Labeling
Selectins
Passive Immunization
Serum
Abdomen
Hypotension

Keywords

  • Ischemia-reperfusion injury
  • Selectin
  • Sulfatide

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Squadrito, F., Altavilla, D., Squadrito, G., Campo, G. M., Arlotta, M., Quartarone, C., ... Caputi, A. P. (1998). Sulfatide reduces leucocyte accumulation and reverts vascular failure in splanchnic artery occlusion shock. European Journal of Pharmacology, 361(1), 101-108. https://doi.org/10.1016/S0014-2999(98)00705-5

Sulfatide reduces leucocyte accumulation and reverts vascular failure in splanchnic artery occlusion shock. / Squadrito, Francesco; Altavilla, Domenica; Squadrito, Giovanni; Campo, Giuseppe M.; Arlotta, Mariarita; Quartarone, Cristina; Minutoli, Letteria; Ferlito, Marcella; Saitta, Antonino; Caputi, Achille P.

In: European Journal of Pharmacology, Vol. 361, No. 1, 13.11.1998, p. 101-108.

Research output: Contribution to journalArticle

Squadrito, F, Altavilla, D, Squadrito, G, Campo, GM, Arlotta, M, Quartarone, C, Minutoli, L, Ferlito, M, Saitta, A & Caputi, AP 1998, 'Sulfatide reduces leucocyte accumulation and reverts vascular failure in splanchnic artery occlusion shock', European Journal of Pharmacology, vol. 361, no. 1, pp. 101-108. https://doi.org/10.1016/S0014-2999(98)00705-5
Squadrito, Francesco ; Altavilla, Domenica ; Squadrito, Giovanni ; Campo, Giuseppe M. ; Arlotta, Mariarita ; Quartarone, Cristina ; Minutoli, Letteria ; Ferlito, Marcella ; Saitta, Antonino ; Caputi, Achille P. / Sulfatide reduces leucocyte accumulation and reverts vascular failure in splanchnic artery occlusion shock. In: European Journal of Pharmacology. 1998 ; Vol. 361, No. 1. pp. 101-108.
@article{1741150e802d4f8a8241f37b3c4c3cc4,
title = "Sulfatide reduces leucocyte accumulation and reverts vascular failure in splanchnic artery occlusion shock",
abstract = "Selectin-mediated leucocyte accumulation is implicated in the pathogenesis of splanchnic artery occlusion. Sulfatide is recognized by P-selectin and blocks this adhesion molecule. We investigated the effects of sulfatide in rats subjected to splanchnic artery occlusion shock. Anaesthetized rats, subjected to total occlusion of the superior mesenteric artery and the coeliac trunk for 45 min developed severe shock resulting in death within 85-90 min after the release of occlusion. Sham operated animals were used as controls. Splanchnic artery occlusion shocked rats had marked hypotension, enhanced levels of tumor necrosis factor-α (TNF-α) in serum and macrophages, leucopenia and increased ileal leucocyte accumulation, studied by the means of myeloperoxidase activity. Furthermore, aortae from shocked rats showed marked hyporeactivity to phenylephrine (1 nM-10 μM), reduced responsiveness to acetylcholine (10 nM-10 μM) and an increased staining for P-selectin in the vasculature. In vivo administration of sulfatide (10 mg/kg, i.v., 5 min after occlusion of the splanchnic arteries increased survival rate (90{\%}, 4 h after splanchnic artery occlusion shock , enhanced mean arterial blood pressure,reduced serum TNF-a 3711 U r ml vs. 39818 U r ml , ameliorated leucopenia and reduced ileal myeloproxidase activity (1.2 ± 0.4 U/g tissue vs. 8.2 ± 0.8 U/g tissue). Aortae from splanchnic artery occlusion shocked rats treated with sulfatide exhibited a greater contractile response to phenylephrine and improved responsiveness to acetylcholine. Moreover sulfatide-treated rats showed a reduced staining for P-selectin in the aorta and in the superior mesenteric artery. Finally, passive immunization with specific monoclonal antibodies raised against P-selectin significantly protected from the lethality induced by splanchnic artery occlusion shock. Our results suggest that sulfatide protects against splanchnic artery occlusion shock. (C) 1998 Elsevier Science B.V. All rights reserved.",
keywords = "Ischemia-reperfusion injury, Selectin, Sulfatide",
author = "Francesco Squadrito and Domenica Altavilla and Giovanni Squadrito and Campo, {Giuseppe M.} and Mariarita Arlotta and Cristina Quartarone and Letteria Minutoli and Marcella Ferlito and Antonino Saitta and Caputi, {Achille P.}",
year = "1998",
month = "11",
day = "13",
doi = "10.1016/S0014-2999(98)00705-5",
language = "English (US)",
volume = "361",
pages = "101--108",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Sulfatide reduces leucocyte accumulation and reverts vascular failure in splanchnic artery occlusion shock

AU - Squadrito, Francesco

AU - Altavilla, Domenica

AU - Squadrito, Giovanni

AU - Campo, Giuseppe M.

AU - Arlotta, Mariarita

AU - Quartarone, Cristina

AU - Minutoli, Letteria

AU - Ferlito, Marcella

AU - Saitta, Antonino

AU - Caputi, Achille P.

PY - 1998/11/13

Y1 - 1998/11/13

N2 - Selectin-mediated leucocyte accumulation is implicated in the pathogenesis of splanchnic artery occlusion. Sulfatide is recognized by P-selectin and blocks this adhesion molecule. We investigated the effects of sulfatide in rats subjected to splanchnic artery occlusion shock. Anaesthetized rats, subjected to total occlusion of the superior mesenteric artery and the coeliac trunk for 45 min developed severe shock resulting in death within 85-90 min after the release of occlusion. Sham operated animals were used as controls. Splanchnic artery occlusion shocked rats had marked hypotension, enhanced levels of tumor necrosis factor-α (TNF-α) in serum and macrophages, leucopenia and increased ileal leucocyte accumulation, studied by the means of myeloperoxidase activity. Furthermore, aortae from shocked rats showed marked hyporeactivity to phenylephrine (1 nM-10 μM), reduced responsiveness to acetylcholine (10 nM-10 μM) and an increased staining for P-selectin in the vasculature. In vivo administration of sulfatide (10 mg/kg, i.v., 5 min after occlusion of the splanchnic arteries increased survival rate (90%, 4 h after splanchnic artery occlusion shock , enhanced mean arterial blood pressure,reduced serum TNF-a 3711 U r ml vs. 39818 U r ml , ameliorated leucopenia and reduced ileal myeloproxidase activity (1.2 ± 0.4 U/g tissue vs. 8.2 ± 0.8 U/g tissue). Aortae from splanchnic artery occlusion shocked rats treated with sulfatide exhibited a greater contractile response to phenylephrine and improved responsiveness to acetylcholine. Moreover sulfatide-treated rats showed a reduced staining for P-selectin in the aorta and in the superior mesenteric artery. Finally, passive immunization with specific monoclonal antibodies raised against P-selectin significantly protected from the lethality induced by splanchnic artery occlusion shock. Our results suggest that sulfatide protects against splanchnic artery occlusion shock. (C) 1998 Elsevier Science B.V. All rights reserved.

AB - Selectin-mediated leucocyte accumulation is implicated in the pathogenesis of splanchnic artery occlusion. Sulfatide is recognized by P-selectin and blocks this adhesion molecule. We investigated the effects of sulfatide in rats subjected to splanchnic artery occlusion shock. Anaesthetized rats, subjected to total occlusion of the superior mesenteric artery and the coeliac trunk for 45 min developed severe shock resulting in death within 85-90 min after the release of occlusion. Sham operated animals were used as controls. Splanchnic artery occlusion shocked rats had marked hypotension, enhanced levels of tumor necrosis factor-α (TNF-α) in serum and macrophages, leucopenia and increased ileal leucocyte accumulation, studied by the means of myeloperoxidase activity. Furthermore, aortae from shocked rats showed marked hyporeactivity to phenylephrine (1 nM-10 μM), reduced responsiveness to acetylcholine (10 nM-10 μM) and an increased staining for P-selectin in the vasculature. In vivo administration of sulfatide (10 mg/kg, i.v., 5 min after occlusion of the splanchnic arteries increased survival rate (90%, 4 h after splanchnic artery occlusion shock , enhanced mean arterial blood pressure,reduced serum TNF-a 3711 U r ml vs. 39818 U r ml , ameliorated leucopenia and reduced ileal myeloproxidase activity (1.2 ± 0.4 U/g tissue vs. 8.2 ± 0.8 U/g tissue). Aortae from splanchnic artery occlusion shocked rats treated with sulfatide exhibited a greater contractile response to phenylephrine and improved responsiveness to acetylcholine. Moreover sulfatide-treated rats showed a reduced staining for P-selectin in the aorta and in the superior mesenteric artery. Finally, passive immunization with specific monoclonal antibodies raised against P-selectin significantly protected from the lethality induced by splanchnic artery occlusion shock. Our results suggest that sulfatide protects against splanchnic artery occlusion shock. (C) 1998 Elsevier Science B.V. All rights reserved.

KW - Ischemia-reperfusion injury

KW - Selectin

KW - Sulfatide

UR - http://www.scopus.com/inward/record.url?scp=0031753937&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031753937&partnerID=8YFLogxK

U2 - 10.1016/S0014-2999(98)00705-5

DO - 10.1016/S0014-2999(98)00705-5

M3 - Article

C2 - 9851547

AN - SCOPUS:0031753937

VL - 361

SP - 101

EP - 108

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 1

ER -