Successful respiratory immunization with dry powder live-attenuated measles virus vaccine in rhesus macaques

Wen Hsuan Lin, Diane E. Griffin, Paul A. Rota, Mark Papania, Stephen P. Cape, David Bennett, Brian Quinn, Robert E. Sievers, Charles Shermer, Kenneth Powell, Robert J. Adams, Steven Godin, Scott Winston

Research output: Contribution to journalArticlepeer-review

Abstract

Measles remains an important cause of childhood mortality worldwide. Sustained high vaccination coverage is the key to preventing measles deaths. Because measles vaccine is delivered by injection, hurdles to high coverage include the need for trained medical personnel and a cold chain, waste of vaccine in multidose vials and risks associated with needle use and disposal. Respiratory vaccine delivery could lower these barriers and facilitate sustained high coverage. We developed a novel single unit dose, dry powder live-attenuated measles vaccine (MVDP) for respiratory delivery without reconstitution. We tested the immunogenicity and protective efficacy in rhesus macaques of one dose of MVDP delivered either with a mask or directly intranasal with two dry powder inhalers, PuffHaler and BD Solovent. MVDP induced robust measles virus (MeV)-specific humoral and T-cell responses, without adverse effects, which completely protected the macaques from infection with wild-type MeV more than one year later. Respiratory delivery of MVDP was safe and effective and could aid in measles control.

Original languageEnglish (US)
Pages (from-to)2987-2992
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number7
DOIs
StatePublished - Feb 15 2011

Keywords

  • Aerosol delivery
  • Antibody avidity
  • Multifunctional T cells
  • Protective immunity

ASJC Scopus subject areas

  • General

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