Successful growth of fresh retinoblastoma cells in chorioallantoic membrane

Silvia L. Fialho; Bárbara R. Silvestrini; Juliana Vieira; Mayara R.B. Paiva; Luciana M. Silva; Fernando Chahud; Armando Silva-Cunha; Zelia M. Correa; Rodrigo Jorge

doi:10.1186/s40942-020-00236-x

Successful growth of fresh retinoblastoma cells in chorioallantoic membrane

Silvia L. Fialho, Bárbara R. Silvestrini, Juliana Vieira, Mayara R.B. Paiva, Luciana M. Silva, Fernando Chahud, Armando Silva-Cunha, Zelia M. Correa, Rodrigo Jorge

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

The authors developed a retinoblastoma model using fresh harvested cells from an enucleated eye that were transplanted in chick embryos (chorioallantoic membrane model). The transplanted embryos were treated with escalating doses of Melphalan. This exploratory model was developed with the goal of testing drug sensitivity. Our findings suggest this tumor model could be employed to personalize treatment for patients with retinoblastoma, especially those with bilateral and more refractory disease.

Original language	English (US)
Article number	33
Journal	International Journal of Retina and Vitreous
Volume	6
Issue number	1
DOIs	https://doi.org/10.1186/s40942-020-00236-x
State	Published - Jul 29 2020

Keywords

CAM assay
Chorioallantoic membrane
Retinoblastoma
Tumor models

ASJC Scopus subject areas

Ophthalmology

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10.1186/s40942-020-00236-x

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title = "Successful growth of fresh retinoblastoma cells in chorioallantoic membrane",

abstract = "The authors developed a retinoblastoma model using fresh harvested cells from an enucleated eye that were transplanted in chick embryos (chorioallantoic membrane model). The transplanted embryos were treated with escalating doses of Melphalan. This exploratory model was developed with the goal of testing drug sensitivity. Our findings suggest this tumor model could be employed to personalize treatment for patients with retinoblastoma, especially those with bilateral and more refractory disease.",

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AU - Fialho, Silvia L.

AU - Silvestrini, Bárbara R.

AU - Vieira, Juliana

AU - Paiva, Mayara R.B.

AU - Silva, Luciana M.

AU - Chahud, Fernando

AU - Silva-Cunha, Armando

AU - Correa, Zelia M.

AU - Jorge, Rodrigo

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AB - The authors developed a retinoblastoma model using fresh harvested cells from an enucleated eye that were transplanted in chick embryos (chorioallantoic membrane model). The transplanted embryos were treated with escalating doses of Melphalan. This exploratory model was developed with the goal of testing drug sensitivity. Our findings suggest this tumor model could be employed to personalize treatment for patients with retinoblastoma, especially those with bilateral and more refractory disease.

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KW - Tumor models

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Successful growth of fresh retinoblastoma cells in chorioallantoic membrane

Abstract

Keywords

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