Successful genome-wide scan in paired blood and buccal samples

Heather Spencer Feigelson, Carmen Rodriguez, Robert Welch, Amy Hutchinson, Wen Shao, Kevin Jacobs, W. Ryan Diver, Eugenia E. Calle, Michael J. Thun, David J. Hunter, Gilles Thomas, Stephen J. Chanock

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Interest in genome-wide association studies to identify susceptibility alleles for cancer is growing, and several are currently planned or under way. Although the feasibility of collecting buccal cell samples as an alternative to venous blood samples as a source of genomic DNA has been shown, the validity of using DNA from buccal cells for genome-wide scans has not been assessed. We used 46 paired buffy coat and buccal cell samples to test the feasibility of using DNA from buccal cells for genotyping with the HumanHap300 Bead Chip (v.1.0.0) on the Illumina Infinium II platform. Genotyping was successful in every sample, regardless of DNA yield or sample type. Of the 317,502 genotypes attempted, 315,314 (99.3%) were successfully called. Completion rates were similar for buffy coat and buccal cell samples (99.63% and 99.44%, respectively; P = 0.15). Completion rates <99% were observed in only four samples and did not differ by specimen type. The paired samples showed exceptionally high concordance (99.96%). These results show that buccal cell samples collected and processed under optimal conditions can be used for genome-wide association studies with results comparable to those obtained from DNA extracted from buffy coat.

Original languageEnglish (US)
Pages (from-to)1023-1025
Number of pages3
JournalCancer Epidemiology Biomarkers and Prevention
Issue number5
StatePublished - May 2007
Externally publishedYes

ASJC Scopus subject areas

  • Epidemiology
  • Oncology


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