Successful fertilization and pregnancy outcome in in-vitro fertilization using cryopreserved/thawed spermatozoa from patients with malignant diseases

E. Khalifa, S. Oehninger, A. A. Acosta, M. Morshedi, L. Veeck, R. G. Bryzyski, S. J. Muasher

Research output: Contribution to journalArticle

Abstract

Cryopreservation of spermatozoa before treatment is the only proven effective method available to circumvent the sterilizing effect of therapy in some patients with malignant diseases. Because of impaired sperm quality after freezing and thawing in-vitro fertilization/embryo transfer (IVF/ET) was indicated in 10 patients (12 cycles) during 1986-1990. The patient's mean age was 33.4 ± 1.6 years. The following diagnoses were made: seminoma (1), testicular carcinoma (3), leiomyosarcoma of the prostate (1), Wegener's granulomatosis (1), non-Hodgkin's (1) and Hodgkin's lymphoma (3). When motile spermatozoa could be recovered after thawing, the total fraction of motile spermatozoa after swim-up separation ranged from 0.2 to 4.2 × 106 spermatozoa/ml (eight patients, nine cycles). In all these cases, insemination was performed with multiple oocytes per dish. Fertilization was achieved when swim-up recovered a mean of 1.8 ± 0.5 × 106 spermatozoa/ml and when insemination was performed with at least a calculated concentration of motile spermatozoa of 1 × 105 spermatozoa/oocyte. The fertilization rate of preovulatory oocytes was 60%. Four patients achieved a pregnancy: two of them delivered a single healthy baby, one delivered triplet healthy babies and one had a preclinical abortion. In two patients (three cycles), no motile spermatozoa were recovered after thawing, and micromanipulation of oocytes for assisted fertilization was performed. Although fertilized oocytes were transferred, those couples did not achieve a pregnancy. Patients with lymphopathies had the best results, whilst those with testicular neoplasms had the poorest outcome, thus suggesting a poor gametogenic function in the non-affected testis. These results give hope to some patients with malignant diseases to maintain their reproductive capacity through sperm banking and IVF/ET.

Original languageEnglish (US)
Pages (from-to)105-108
Number of pages4
JournalHuman Reproduction
Volume7
Issue number1
StatePublished - Jan 1992
Externally publishedYes

Fingerprint

Spermatozoa
In Vitro Fertilization
Thawing
Fertilization
Pregnancy
Fertilization in Vitro
Pregnancy Outcome
Oocyte
Oocytes
Embryo Transfer
Freezing
Cycle
Insemination
Cryopreservation
Micromanipulation
In vitro fertilization
Banking
Seminoma
Granulomatosis with Polyangiitis
Leiomyosarcoma

Keywords

  • Embryo transfer
  • IVF
  • Neoplastic disease
  • Sperm cryopreservation

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Rehabilitation
  • Developmental Biology
  • Physiology

Cite this

Khalifa, E., Oehninger, S., Acosta, A. A., Morshedi, M., Veeck, L., Bryzyski, R. G., & Muasher, S. J. (1992). Successful fertilization and pregnancy outcome in in-vitro fertilization using cryopreserved/thawed spermatozoa from patients with malignant diseases. Human Reproduction, 7(1), 105-108.

Successful fertilization and pregnancy outcome in in-vitro fertilization using cryopreserved/thawed spermatozoa from patients with malignant diseases. / Khalifa, E.; Oehninger, S.; Acosta, A. A.; Morshedi, M.; Veeck, L.; Bryzyski, R. G.; Muasher, S. J.

In: Human Reproduction, Vol. 7, No. 1, 01.1992, p. 105-108.

Research output: Contribution to journalArticle

Khalifa, E, Oehninger, S, Acosta, AA, Morshedi, M, Veeck, L, Bryzyski, RG & Muasher, SJ 1992, 'Successful fertilization and pregnancy outcome in in-vitro fertilization using cryopreserved/thawed spermatozoa from patients with malignant diseases', Human Reproduction, vol. 7, no. 1, pp. 105-108.
Khalifa, E. ; Oehninger, S. ; Acosta, A. A. ; Morshedi, M. ; Veeck, L. ; Bryzyski, R. G. ; Muasher, S. J. / Successful fertilization and pregnancy outcome in in-vitro fertilization using cryopreserved/thawed spermatozoa from patients with malignant diseases. In: Human Reproduction. 1992 ; Vol. 7, No. 1. pp. 105-108.
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abstract = "Cryopreservation of spermatozoa before treatment is the only proven effective method available to circumvent the sterilizing effect of therapy in some patients with malignant diseases. Because of impaired sperm quality after freezing and thawing in-vitro fertilization/embryo transfer (IVF/ET) was indicated in 10 patients (12 cycles) during 1986-1990. The patient's mean age was 33.4 ± 1.6 years. The following diagnoses were made: seminoma (1), testicular carcinoma (3), leiomyosarcoma of the prostate (1), Wegener's granulomatosis (1), non-Hodgkin's (1) and Hodgkin's lymphoma (3). When motile spermatozoa could be recovered after thawing, the total fraction of motile spermatozoa after swim-up separation ranged from 0.2 to 4.2 × 106 spermatozoa/ml (eight patients, nine cycles). In all these cases, insemination was performed with multiple oocytes per dish. Fertilization was achieved when swim-up recovered a mean of 1.8 ± 0.5 × 106 spermatozoa/ml and when insemination was performed with at least a calculated concentration of motile spermatozoa of 1 × 105 spermatozoa/oocyte. The fertilization rate of preovulatory oocytes was 60{\%}. Four patients achieved a pregnancy: two of them delivered a single healthy baby, one delivered triplet healthy babies and one had a preclinical abortion. In two patients (three cycles), no motile spermatozoa were recovered after thawing, and micromanipulation of oocytes for assisted fertilization was performed. Although fertilized oocytes were transferred, those couples did not achieve a pregnancy. Patients with lymphopathies had the best results, whilst those with testicular neoplasms had the poorest outcome, thus suggesting a poor gametogenic function in the non-affected testis. These results give hope to some patients with malignant diseases to maintain their reproductive capacity through sperm banking and IVF/ET.",
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