Subversion of interleukin-1-mediated host defence by a nasal carrier strain of Staphylococcus aureus

Gerry A. Quinn, Patrick Tarwater, Alexander M. Cole

Research output: Contribution to journalArticle

Abstract

Staphylococcus aureus, a major source of nosocomial and community-acquired infections, has a nasal carriage rate exceeding 25% in the human population. To elucidate host-pathogen interactions pertaining to nasal carriage, we examined the role of interleukin-1 (IL-1) in the colonization of human nasal epithelial cells (NEC) by a nasal carrier strain and a non-carrier strain of S. aureus. Using an organotypic model of the nasal epithelium, we observed that inoculation with a non-carrier strain of S. aureus induced production of IL-1 from NEC, but the expression of this cytokine was significantly reduced when NEC were inoculated with a carrier strain. Moreover, both IL-1α and IL-1β significantly decreased the growth of the nasal carrier strain of S. aureus (P < 0·001, n = 17 to n = 25); however the growth of the non-carrier strain was unaffected. Interestingly, it was found that several nasal carrier strains of S. aureus form quorum-dependent biofilms, which can be partially inhibited when preincubated with IL-1α. Taken together these data suggest that, although nasal carrier strains of S. aureus are sensitive to IL-1, they display a significant colonization advantage by both preventing the host from expressing IL-1 and elaborating a protective biofilm.

Original languageEnglish (US)
JournalImmunology
Volume128
Issue number1 PART 2
DOIs
Publication statusPublished - Sep 1 2009
Externally publishedYes

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Keywords

  • Bacteria/bacterial immunity
  • Cytokines
  • Innate immunity

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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