TY - JOUR
T1 - Subventricular zone localized irradiation affects the generation of proliferating neural precursor cells and the migration of neuroblasts
AU - Achanta, Pragathi
AU - Capilla-Gonzalez, Vivian
AU - Purger, David
AU - Reyes, Juvenal
AU - Sailor, Kurt
AU - Song, Hongjun
AU - Garcia-Verdugo, Jose Manuel
AU - Gonzalez-Perez, Oscar
AU - Ford, Eric
AU - Quinones-Hinojosa, Alfredo
PY - 2012/11
Y1 - 2012/11
N2 - Radiation therapy is a part of the standard treatment for brain tumor patients, often resulting in irreversible neuropsychological deficits. These deficits may be due to permanent damage to the neural stem cell (NSC) niche, damage to local neural progenitors, or neurotoxicity. Using a computed tomography-guided localized radiation technique, we studied the effects of radiation on NSC proliferation and neuroblast migration in the mouse brain. Localized irradiation of the subventricular zone (SVZ) eliminated the proliferating neural precursor cells and migrating neuroblasts. After irradiation, type B cells in the SVZ lacked the ability to generate migrating neuroblasts. Neuroblasts from the unirradiated posterior SVZ did not follow their normal migratory path through the irradiated anterior SVZ. Our results indicate that the migrating neuroblasts were not replenished, despite the presence of type B cells in the SVZ post-irradiation. This study provides novel insights into the effects of localized SVZ radiation on neurogenesis and cell migration that may potentially lead to the development of new radiotherapy strategies to minimize damage to NSCs and neuroblast migration.
AB - Radiation therapy is a part of the standard treatment for brain tumor patients, often resulting in irreversible neuropsychological deficits. These deficits may be due to permanent damage to the neural stem cell (NSC) niche, damage to local neural progenitors, or neurotoxicity. Using a computed tomography-guided localized radiation technique, we studied the effects of radiation on NSC proliferation and neuroblast migration in the mouse brain. Localized irradiation of the subventricular zone (SVZ) eliminated the proliferating neural precursor cells and migrating neuroblasts. After irradiation, type B cells in the SVZ lacked the ability to generate migrating neuroblasts. Neuroblasts from the unirradiated posterior SVZ did not follow their normal migratory path through the irradiated anterior SVZ. Our results indicate that the migrating neuroblasts were not replenished, despite the presence of type B cells in the SVZ post-irradiation. This study provides novel insights into the effects of localized SVZ radiation on neurogenesis and cell migration that may potentially lead to the development of new radiotherapy strategies to minimize damage to NSCs and neuroblast migration.
KW - Migrating neuroblasts
KW - Neural stem cells
KW - Radiation therapy
KW - Subventricular zone
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U2 - 10.1002/stem.1214
DO - 10.1002/stem.1214
M3 - Article
C2 - 22948813
AN - SCOPUS:84867909513
SN - 1066-5099
VL - 30
SP - 2548
EP - 2560
JO - Stem Cells
JF - Stem Cells
IS - 11
ER -