Subunit structures of different electrophoretic forms of nucleosomes.

S. C. Albright, J. M. Wiseman, R. A. Lange, W. T. Garrard

Research output: Contribution to journalArticle

Abstract

We have reported previously that five different electrophoretic forms of mononucleosomes (MI to MV) are produced upon treatment of mammalian chromatin with micrococcal nuclease. We show here that each of these mononucleosome classes possesses internal heterogeneity due to the presence of a variety of minor protein species. Defined subsets of mononucleosome classes MII to MV have been reconstituted by reassociating stripped nucleosomes with histone H1 and non-histone protein HMG-17. This procedure leads to the generation of the same five major electrophoretic forms of mononucleosomes found in native chromatin. From the results of one- and two-dimensional electrophoretic analyses on reconstituted samples, it is concluded that different mononucleosome classes possess the following subunit structures: MI, core histone octamer (8-mer); MII, 8-mer plus one copy of HMG-17; MIIIA, 8-mer plus one copy of histone H1; MIIIB, 8-mer plus two copies of HMG-17; MIV, 8-mer plus one copy each of histone H1 and HMG-17; and MV, 8-mer plus one copy of histone H1 and two copies of HMG-17. Equal numbers of HMG-14 molecules can substitute for HMG-17 and generate the same nucleosome components. Thus, mononucleosomes possess independent binding sites for at least 1 histone H1 molecule and 2 nonhistone chromosomal protein molecules. We show further that reassociated HMG-17 molecules can exhibit a rapid interchange between binding sites, even under conditions of low ionic strength.

Original languageEnglish (US)
Pages (from-to)3673-3684
Number of pages12
JournalJournal of Biological Chemistry
Volume255
Issue number8
StatePublished - Apr 25 1980
Externally publishedYes

Fingerprint

HMGN2 Protein
Nucleosomes
Histones
Molecules
Chromatin
HMGN1 Protein
Non Histone Chromosomal Proteins
Binding Sites
Micrococcal Nuclease
Interchanges
Ionic strength
Osmolar Concentration

ASJC Scopus subject areas

  • Biochemistry

Cite this

Albright, S. C., Wiseman, J. M., Lange, R. A., & Garrard, W. T. (1980). Subunit structures of different electrophoretic forms of nucleosomes. Journal of Biological Chemistry, 255(8), 3673-3684.

Subunit structures of different electrophoretic forms of nucleosomes. / Albright, S. C.; Wiseman, J. M.; Lange, R. A.; Garrard, W. T.

In: Journal of Biological Chemistry, Vol. 255, No. 8, 25.04.1980, p. 3673-3684.

Research output: Contribution to journalArticle

Albright, SC, Wiseman, JM, Lange, RA & Garrard, WT 1980, 'Subunit structures of different electrophoretic forms of nucleosomes.', Journal of Biological Chemistry, vol. 255, no. 8, pp. 3673-3684.
Albright SC, Wiseman JM, Lange RA, Garrard WT. Subunit structures of different electrophoretic forms of nucleosomes. Journal of Biological Chemistry. 1980 Apr 25;255(8):3673-3684.
Albright, S. C. ; Wiseman, J. M. ; Lange, R. A. ; Garrard, W. T. / Subunit structures of different electrophoretic forms of nucleosomes. In: Journal of Biological Chemistry. 1980 ; Vol. 255, No. 8. pp. 3673-3684.
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