Subunit-specific effects of isoflurane on neuronal Ih in HCN1 knockout mice

Xiangdong Chen, Shaofang Shu, Dylan P. Kennedy, Sarah C. Willcox, Douglas A. Bayliss

Research output: Contribution to journalArticle

Abstract

The ionic mechanisms that contribute to general anesthetic actions have not been elucidated, although increasing evidence has pointed to roles for subthreshold ion channels, such as the HCN channels underlying the neuronal hyperpolarization-activated cationic current (Ih). Here, we used conventional HCN1 knockout mice to test directly the contributions of specific HCN subunits to effects of isoflurane, an inhalational anesthetic, on membrane and integrative properties of motor and cortical pyramidal neurons in vitro. Compared with wild-type mice, residual Ih from knockout animals was smaller in amplitude and presented with HCN2-like properties. Inhibition of Ih by isoflurane previously attributed to HCN1 subunit-containing channels (i.e., a hyperpolarizing shift in half-activation voltage [V 1/2]) was absent in neurons from HCN1 knockout animals; the remaining inhibition of current amplitude could be attributed to effects on residual HCN2 channels. We also found that isoflurane increased temporal summation of excitatory postsynaptic potentials (EPSPs) in cortical neurons from wild-type mice; this effect was predicted by simulation of anesthetic-induced dendritic Ih inhibition, which also revealed more prominent summation accompanying shifts in V1/2 (an HCN1-like effect) than decreased current amplitude (an HCN2-like effect). Accordingly, anesthetic-induced EPSP summation was not observed in cortical cells from HCN1 knockout mice. In wild-type mice, the enhanced synaptic summation observed with low concentrations of isoflurane contributed to a net increase in cortical neuron excitability. In summary, HCN channel subunits account for distinct anesthetic effects on neuronal membrane properties and synaptic integration; inhibition of HCN1 in cortical neurons may contribute to the synaptically mediated slow-wave cortical synchronization that accompanies anesthetic-induced hypnosis.

Original languageEnglish (US)
Pages (from-to)129-140
Number of pages12
JournalJournal of Neurophysiology
Volume101
Issue number1
DOIs
StatePublished - Jan 2009
Externally publishedYes

Fingerprint

Isoflurane
Knockout Mice
Anesthetics
Neurons
Excitatory Postsynaptic Potentials
Anesthetic Hypnosis
Postsynaptic Potential Summation
Cortical Synchronization
Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
Synaptic Membranes
General Anesthetics
Pyramidal Cells
Ion Channels
Membranes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Cite this

Chen, X., Shu, S., Kennedy, D. P., Willcox, S. C., & Bayliss, D. A. (2009). Subunit-specific effects of isoflurane on neuronal Ih in HCN1 knockout mice. Journal of Neurophysiology, 101(1), 129-140. https://doi.org/10.1152/jn.01352.2007

Subunit-specific effects of isoflurane on neuronal Ih in HCN1 knockout mice. / Chen, Xiangdong; Shu, Shaofang; Kennedy, Dylan P.; Willcox, Sarah C.; Bayliss, Douglas A.

In: Journal of Neurophysiology, Vol. 101, No. 1, 01.2009, p. 129-140.

Research output: Contribution to journalArticle

Chen, X, Shu, S, Kennedy, DP, Willcox, SC & Bayliss, DA 2009, 'Subunit-specific effects of isoflurane on neuronal Ih in HCN1 knockout mice', Journal of Neurophysiology, vol. 101, no. 1, pp. 129-140. https://doi.org/10.1152/jn.01352.2007
Chen, Xiangdong ; Shu, Shaofang ; Kennedy, Dylan P. ; Willcox, Sarah C. ; Bayliss, Douglas A. / Subunit-specific effects of isoflurane on neuronal Ih in HCN1 knockout mice. In: Journal of Neurophysiology. 2009 ; Vol. 101, No. 1. pp. 129-140.
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