Subunit complementation of Escherichia coli adenylosuccinate synthetase

Chulhun Kang, Sangwon Kim, Herbert J. Fromm

Research output: Contribution to journalArticle

Abstract

Data are presented, based upon subunit complementation experiments, that suggest that Escherichia coli adenylosuccinate synthetase contains two shared active sites between its dimeric interface. This conclusion was alluded to by use of mutant forms of adenylosuccinate synthetase previously prepared by site-directed mutagenesis. The experiments indicate that, although the R143L and D13A mutants have low or no activity independently, when they are mixed, a significant amount of activity was obtained. These results indicate that the subunits exchange with each other to form heterodimers with a single viable wild-type active site. The k(cat) value for the active hybrid active site in the R143L-D13A heterodimer is virtually identical to that observed with the wild-type enzyme, and the other kinetic parameters are very similar to those found for the wild-type enzyme. An analysis of the restoration of the activity in the presence of substrates suggests that GTP and IMP stabilize the dimeric structure of the protein. A comparison of the restoration of the activity using different combinations of mutants provides evidence indicating that some of the GTP binding elements, including the P- loop, in the protein are important for subunit integrity. Also, for the first time, a comprehensive analysis of subunit complementation is performed for the two inactive mutants (R143L and D13A) where the dissociation constants for the R143L-D13A heterodimer and the D13A homodimer were determined to be 21 and 2.9 μM, respectively. A concentration dependence of the specific activity of the wild-type protein in this study shows that the K(d) for dimer dissociation is approximately 1 μM.

Original languageEnglish (US)
Pages (from-to)29722-29728
Number of pages7
JournalJournal of Biological Chemistry
Volume271
Issue number47
DOIs
StatePublished - 1996
Externally publishedYes

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Adenylosuccinate Synthase
Escherichia coli
Catalytic Domain
Guanosine Triphosphate
Restoration
Inosine Monophosphate
Mutagenesis
Proteins
Protein Subunits
Enzymes
Site-Directed Mutagenesis
Kinetic parameters
Dimers
Experiments
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Subunit complementation of Escherichia coli adenylosuccinate synthetase. / Kang, Chulhun; Kim, Sangwon; Fromm, Herbert J.

In: Journal of Biological Chemistry, Vol. 271, No. 47, 1996, p. 29722-29728.

Research output: Contribution to journalArticle

Kang, Chulhun ; Kim, Sangwon ; Fromm, Herbert J. / Subunit complementation of Escherichia coli adenylosuccinate synthetase. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 47. pp. 29722-29728.
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