Subtracted differential display: Genes with amphetamine-altered expression patterns include calcineurin

Xiao Bing Wang, George R. Uhl

Research output: Contribution to journalArticlepeer-review

Abstract

Genes whose expression changes with administration of abused substances provide candidate biochemical mechanisms for drug-induced long-term brain changes. To identify such genes, and to avoid the false positive results frequently obtained from differential display PCR, we applied a subtracted differential display (SDD) approach. We subtracted single-stranded cDNA prepared from drug-treated animals with excess mRNA from saline-treated animals, and visa versa, prior to differential display amplifications. Two of the initial amphetamine-regulated cDNAs identified in this fashion encoded calcineurin A, a neuron-specific protein phosphatase catalytic subunit whose striatal expression was upregulated ca. 1.5-fold. SDD may enhance the utility of differential display approaches to identifying regulated genes in tissues in which mRNA complexities are high.

Original languageEnglish (US)
Pages (from-to)344-347
Number of pages4
JournalMolecular Brain Research
Volume53
Issue number1-2
DOIs
StatePublished - Jan 1998

Keywords

  • Amphetamine
  • Drug abuse
  • Gene regulation
  • PCR differential display
  • Subtraction

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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